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Catalog Products » Antibodies » Primary Antibodies » Cytokine and Growth Factor Antibodies » IP-10 Antibody, mAb, Mouse

IP-10 Antibody, mAb, Mouse

Synonyms Interferon-γ-Inducible Protein-10 human; CXCL10
Description Mouse Anti-IP-10 Monoclonal Antibody reacts with recombinant human IP-10 fusion protein. The immunogen used to produce Mouse Anti IP-10 antibody was recombinant human IP-10 expressed in PET32a produced in E. coli.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Purified recombinant human IP-10 expressed in PET32a
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein G affinity column

Subclass Mouse IgG2b
Clone ID 4G12D7
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Species Reactivity Human,Reactivity to other species is not tested yet.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0-2.0 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)

IP-10 Antibody, MAb, Mouse
IP-10 Antibody, MAb, Mouse

Lane 1-2: 200 ng, 50 ng purified rHuIP10
Primary antibody: 1 µg/ml Mouse Anti-IP-10 Monoclonal Antibody (GenScript, A00163)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)




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Cat. No. A00163
Size
Price
500 ug $599.00
100 ug $199.00
 
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