×
Catalog Products » Antibodies » THE™ Elite Antibodies » THE™ HA-tag mAb » HA-tag Antibody, pAb, Goat

HA-tag Antibody, pAb, Goat

HA-tag Antibody, PAb, Goat

Western blot analysis of HA-tagged fusion protein using HA-tag Antibody, pAb, Goat (GenScript, A00168, 1 µg/ml)
The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

HA-tag Antibody, PAb, Goat

Western blot analysis of HA tagged fusion protein using 1 μg/ml Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168)
Lane 1-8: N-terminal or C-terminal HA tagged fusion protein cell lysate (≈ 31 kD)
Secondary antibody: Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

HA-tag Antibody, PAb, Goat

Western blot analysis of immunoprecipitates from HA fusion protein lysates using Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168).
Lane 1: Input control material for HA fusion protein lysates
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01009)
Lane 3: Immunprecipitation with Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168).

Synonyms Goat Anti HA-tag; Anti HA-tag
Description Goat Anti-HA-tag Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography.
Host Species Goat
Conjugation Unconjugated
Immunogen YPYDVPDYA (influenza hemagglutinin-HA (epitope)) conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity This antibody specifically reacts with HA tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescence (IF): 2-10 µg/ml
Immunoprecipitation (IP): 2-10 µg/mg of lysate
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

HA-tag Antibody, PAb, Goat
HA-tag Antibody, PAb, Goat

Western blot analysis of HA-tagged fusion protein using HA-tag Antibody, pAb, Goat (GenScript, A00168, 1 µg/ml)
The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

HA-tag Antibody, PAb, Goat
HA-tag Antibody, PAb, Goat

Western blot analysis of HA tagged fusion protein using 1 μg/ml Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168)
Lane 1-8: N-terminal or C-terminal HA tagged fusion protein cell lysate (≈ 31 kD)
Secondary antibody: Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

HA-tag Antibody, PAb, Goat
HA-tag Antibody, PAb, Goat

Western blot analysis of immunoprecipitates from HA fusion protein lysates using Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168).
Lane 1: Input control material for HA fusion protein lysates
Lane 2: Negative control – IP with isotype control antibody (GenScript, A01009)
Lane 3: Immunprecipitation with Goat Anti-HA-tag Polyclonal Antibody (GenScript, A00168).


Cat. No.
Product Name
Size
Price
Quantity
A00168-40 HA-tag Antibody, pAb, Goat
40 ug
$99.00
A00169-100 HA-tag Antibody [HRP], pAb, Goat
100 ug
$199.00
A00169-40 HA-tag Antibody [HRP], pAb, Goat
40 ug
$99.00
A00203-40 HA-tag Antibody [Biotin], pAb, Goat
40 ug
$99.00
A01244-100 THETM HA Tag Antibody, mAb, Mouse
100 ug
$287.00
A01296 THETM HA Tag Antibody [HRP], mAb, Mouse
100 ug
$299.00
A01297 THETM HA Tag Antibody [Biotin], mAb, Mouse
100 ug
$299.00
A01621 THETM HA Tag Antibody [FITC], mAb, Mouse
100 ug
$299.00
A01806-100 THETM HA Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$344.00
A01807-100 THETM HA Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$344.00
A01808-100 THETM HA Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$344.00
 
Lee TV.,et al. Negative regulation of notch signaling by xylose.PLoS Genet.2013Jun;9(6):e1003547

Bashar Ghoujal.,et al. ESCRT‐II's involvement in HIV‐1 genomic RNA trafficking and assembly.Biol Cell.2012Dec;104(12):706-21

Fuguo Wu.,et al. Onecut 1 and Onecut 2 are potential regulators of mouse retinal development.J Comp Neurol.2012Apr;520(5):952-69

Li HY.,et al. Hyperphosphorylation as a defense mechanism to reduce TDP-43 aggregation.PLoS One.2011Aug;6(8):e23075

Chandrasekharan MB.,et al. Decoding the trans-histone crosstalk: Methods to analyze H2B ubiquitination, H3 methylation and their regulatory factors.Methods.2011Jul;54(3):304-14

Arun Anantharam.,et al. A new role for the dynamin GTPase in the regulation of fusion pore expansion.Mol Biol Cell.2011Jun;22(11):1907 - 1918

D Liu, et al. eEF1Bγ is a positive regulator of NF-кB signaling pathway. Biochem Biophys Res Commun. 2014Mar.;

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A00168
Size
Price
40 ug $99.00
24 hour
 
*
*
*
*