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Catalog Products » Antibodies » THE™ Elite Antibodies » THE™ HA-tag mAb » DYKDDDDK-tag Antibody, pAb, Rabbit

DYKDDDDK-tag Antibody, pAb, Rabbit

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 49 kD
Observed Size: 49 kD

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

DYKDDDDK-tag Antibody, PAb, Rabbit

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)

Synonyms Rabbit Anti-DYKDDDDK-tag pAb; Rabbit Anti-Flag-tag pAb;
Description The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Host Species Rabbit
Conjugation Unconjugated
Immunogen A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity This Antibody recognizes DYKDDDDK tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.01-0.05 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunofluorescence:5-20 µg/ml
Flow Cytometry:1-3 µg for 1 x 106 cells
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

DYKDDDDK-tag Antibody, PAb, Rabbit
DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 49 kD
Observed Size: 49 kD

DYKDDDDK-tag Antibody, PAb, Rabbit
DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

DYKDDDDK-tag Antibody, PAb, Rabbit
DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

DYKDDDDK-tag Antibody, PAb, Rabbit
DYKDDDDK-tag Antibody, PAb, Rabbit

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)


Li T.,et al. Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal.J Biol Chem.2013Dec;

Wei Huang.,et al. The N-Terminal Phosphodegron Targets Taz/Wwtr1 Protein For ScfΒ-Trcp-Dependent Degradation In Response To Phosphatidylinositol 3-Kinase Inhibition.J Biol Chem.2012Jul;287(31):26245 - 26253

Xin Zhou.,et al. Targeted polyubiquitylation of RASSF1C by the Mule and SCFbeta-TrCP ligases in response to DNA damage.Biochem J.2012Jan;441(1):227-36

Chen-Ying Liu.,et al. PP1 Cooperates with ASPP2 to Dephosphorylate and Activate TAZ.J Biol Chem.2011Feb;286(7):5558 - 5566

A Reuben, et al. The wild‐type hemochromatosis protein HFE inhibits CD8+ T‐lymphocyte activation. Eur J Immunol. 2014Feb.;

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Cat. No. A00170
Size
Price
40 ug $99.00
24 hour
 
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