Catalog Products » Antibodies » Epitope Tag Antibodies » DYKDDDDK-tag Antibody, pAb, Rabbit
DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 49 kD
Observed Size: 49 kD

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

DYKDDDDK-tag Antibody, PAb, Rabbit

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

DYKDDDDK-tag Antibody, PAb, Rabbit

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)

DYKDDDDK-tag Antibody, pAb, Rabbit

This Antibody recognizes DYKDDDDK tagged fusion proteins.
A00170
$102.00

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Overview
Specificity This Antibody recognizes DYKDDDDK tagged fusion proteins.
Host Species Rabbit
Immunogen A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.05 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000
Immunocytochemistry/Immunofluorescence (ICC/IF) 5-20 µg/ml
Flow Cytometry 1-3 µg for 1 x 106 cells

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable

Examples
  • DYKDDDDK-tag Antibody, PAb, Rabbit
  • DYKDDDDK-tag Antibody, PAb, Rabbit

    Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 49 kD
    Observed Size: 49 kD

  • DYKDDDDK-tag Antibody, PAb, Rabbit
  • DYKDDDDK-tag Antibody, PAb, Rabbit

    Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
    The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
    Predicted Size: 52 kD
    Observed Size: 52 kD

  • DYKDDDDK-tag Antibody, PAb, Rabbit
  • DYKDDDDK-tag Antibody, PAb, Rabbit

    Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

  • DYKDDDDK-tag Antibody, PAb, Rabbit
  • DYKDDDDK-tag Antibody, PAb, Rabbit

    Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)


Background
Target Background The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.It has been used extensively as a general epitope tag in expression vectors.DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Synonyms Rabbit Anti-DYKDDDDK-tag pAb;Rabbit Anti-Flag-tag pAb;

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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