DYKDDDDK-tag Antibody, pAb, Rabbit

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Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 49 kD
Observed Size: 49 kD

Western blot analysis of DYKDDDDK-tagged fusion protein using DYKDDDDK-tag Antibody, pAb, Rabbit (GenScript, A00170, 1 µg/ml)
The signal was developed with One-Step Western^TM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of DYKDDDDK tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody (GenScript, A00170, 1 µg/ml) The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)

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Overview

Synonyms	Rabbit Anti-DYKDDDDK-tag pAb; Rabbit Anti-Flag-tag pAb;
Description	The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. DYKDDDDK-tag Antibody, pAb, Rabbit can be used to detect DYKDDDDK-tagged proteins.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	This Antibody recognizes DYKDDDDK tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.01-0.05 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunofluorescence:5-20 µg/ml
Flow Cytometry:1-3 µg for 1 x 10⁶ cells
Other applications: user-optimized

Examples

Immunofluorescent analysis of HEK293 cells transfected with Flag-tagged CD4 protein using DYKDDDDK-tag Antibody (GenScript, A00170, 10 μg/ml)

Related Products

Cat. No.	Product Name	Price
RP10586-1	DYKDDDDK Peptide - 1 mg	$34.00
L00455W	DYKDDDDK Tag Antibody Plate (White, 96-well) - 5 plates	$229.00
L00455C	DYKDDDDK Tag Antibody Plate (Clear, 8X12 strip) - 5 plates	$229.00
L00455B	DYKDDDDK Tag Antibody Plate (Black, 96-well) - 5 plates	$229.00
L00432-1	Anti-DYKDDDDK G1 Affinity Resin - 1 ml	$281.00
L00432-25	Anti-DYKDDDDK G1 Affinity Resin - 25 ml	$2591.00
L00432-5	Anti-DYKDDDDK G1 Affinity Resin - 5 ml	$663.00
L00432-10	Anti-DYKDDDDK G1 Affinity Resin - 10 ml	$1193.00
L00425	Anti-DYKDDDDK IP Resin - 1 ml	$199.00
A01811-100	THE^TM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse - 100 ug	$167.00
A01810-100	THE^TM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse - 100 ug	$167.00
A01809-100	THE^TM DYKDDDDK Tag Antibody [iFluor 488], mAb, Mouse - 100 ug	$167.00
A01632	THE^TM DYKDDDDK Tag Antibody [FITC], mAb, Mouse - 100 ug	$149.00
A01429-100	THE^TM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 ug	$149.00
A01428-100	THE^TM DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 ug	$149.00
A00187-100	THE^TM DYKDDDDK Tag Antibody, mAb, Mouse - 100 ug	$139.00
A00187-200	THE^TM DYKDDDDK Tag Antibody, mAb, Mouse - 200 ug	$256.00
A00170-40	DYKDDDDK-tag Antibody, pAb, Rabbit - 40 ug	$99.00
A00098	Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb - 1 mg	$79.00

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Li T.,et al. Glyceraldehyde-3-phosphate Dehydrogenase Is Activated by Lysine 254 Acetylation in Response to Glucose Signal.J Biol Chem.2013Dec;

Wei Huang.,et al. The N-Terminal Phosphodegron Targets Taz/Wwtr1 Protein For ScfΒ-Trcp-Dependent Degradation In Response To Phosphatidylinositol 3-Kinase Inhibition.J Biol Chem.2012Jul;287(31):26245 - 26253

Xin Zhou.,et al. Targeted polyubiquitylation of RASSF1C by the Mule and SCFbeta-TrCP ligases in response to DNA damage.Biochem J.2012Jan;441(1):227-36

Chen-Ying Liu.,et al. PP1 Cooperates with ASPP2 to Dephosphorylate and Activate TAZ.J Biol Chem.2011Feb;286(7):5558 - 5566

A Reuben, et al. The wild‐type hemochromatosis protein HFE inhibits CD8+ T‐lymphocyte activation. Eur J Immunol. 2014Feb.;

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