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c-Myc-tag Antibody, pAb, Rabbit

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Western blot analysis of c-Myc fusion protein (MW≈52KD) using 1 µg/ml Rabbit Anti-c-Myc-tag Polyclonal Antibody (GenScript, A00172)
Lane 1-3: c-Myc fusion protein in HEK293 cell lysate
Lane 4: Negative HEK293 cell lysate
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:20,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Western blot analysis of c-Myc tagged fusion proteins expressed in E. coli cell lysate using c-Myc-tag Antibody (GenScript, A00172, 1 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

Customer Reviews:
c-Myc-tag Antibody, pAb, Rabbit (GenScript，A00172) were used in western blotting to show overexpression of Bik1, Pac1, or Dyn1 by GAL1 promoter in budding yeast.
Left and middle:
Western blot of cell lysates prepared from strains with either endogenous or GAL1 promoter driving expression of indicated 13myc-tagged gene.
Right:
Western blot of Dyn1-13myc protein in strains with either the endogenous or GAL1 promoter driving the expression of PAC1 or BIK1.
For all blots, equal amount of total cell lysate was loaded in each lane, transferred to PVDF and probed with anti-c-Myc antibody(GenScript, A00172).
All strains were grown to mid-log phase in SD media supplemented with 2% galactose, and then harvested.

Western blot analysis of immunoprecipitates from c-Myc-tagged protein using c-Myc-tag Antibody, pAb, Rabbit (Cat. No. A00172).
Lane 1: c-Myc tagged fusion protein as control.
Lane 2: Immunoprecipitates of c-Myc tagged fusion protein incubated with Rabbit IgG Control (GenScript, Cat. No. A01008) and Protein A served as negative control
Lane 3: Immunoprecipitates of c-Myc tagged fusion protein incubated with c-Myc-tag Antibody, pAb, Rabbit (GenScript, A00172) and Protein A.

The signal was developed with IRDyeTM800 Conjugated affinity Purified Goat Anti-Rabbit IgG.

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Overview

Synonyms	Rabbit Anti-c-Myc-tag pAb;
Description	The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
Specificity	This Antibody recognizes c-Myc tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Immunofluorescence: 5-20 µg/ml
Immunoprecipitation (IP): 2-5 µg/ml
Other applications: user-optimized

Examples

Related Products

Cat. No.	Product Name	Price
A00172-40	c-Myc-tag Antibody, pAb, Rabbit - 40 ug	$99.00
A00173-40	c-Myc-tag Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
A00704-100	THE^TM c-Myc Tag Antibody, mAb, Mouse - 100 ug	$175.00
A00864-40	THE^TM c-Myc Antibody [Biotin], mAb, Mouse - 40 ug	$74.00
A00864-200	THE^TM c-Myc Antibody [Biotin], mAb, Mouse - 200 ug	$333.00
A00863-100	THE^TM c-Myc Antibody [HRP], mAb, Mouse - 100 ug	$180.00

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