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c-Myc-tag Antibody [HRP], pAb, Rabbit

Synonyms Anti-c-Myc-tag [HRP];
Description Anti-c-Myc-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting c-Myc-tag fusion proteins.
Host Species Rabbit
Conjugation Horseradish Peroxidase (HRP)
Immunogen c-Myc epitope tag peptide EQKLISEEDL conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity This Antibody recognizes c-Myc tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

C-Myc-tag Antibody [HRP], PAb, Rabbit
C-Myc-tag Antibody [HRP], PAb, Rabbit

Western blot analysis of c-Myc -tagged fusion protein using c-Myc-tag Antibody [HRP], pAb, Rabbit (GenScript, A00173, 0.2 µg/ml )
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

Cat. No.
Product Name
A00172-40 c-Myc-tag Antibody, pAb, Rabbit
40 ug
A00173-100 c-Myc-tag Antibody [HRP], pAb, Rabbit
100 ug
A00173-40 c-Myc-tag Antibody [HRP], pAb, Rabbit
40 ug
A00704-100 THETM c-Myc Tag Antibody, mAb, Mouse
100 ug
A00864-40 THETM c-Myc Antibody [Biotin], mAb, Mouse
40 ug
A00864-200 THETM c-Myc Antibody [Biotin], mAb, Mouse
200 ug
A00863-100 THETM c-Myc Antibody [HRP], mAb, Mouse
100 ug

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Cat. No. A00173
40 ug $99.00
100 ug $199.00
24 hour