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Catalog Products » Antibodies » Epitope Tag Antibodies » His-tag Antibody, pAb, Rabbit

His-tag Antibody, pAb, Rabbit

Synonyms His-tag Antibody, pAb, Rabbit;
Description His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen His epitope tag peptide HHHHHH conjugated to KLH
Purification Immunoaffinity chromatography

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags. For N-terminal His-tagged protein detection, THETM Anti-His mAb (Cat. No. A00186) is recommended.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized


Cat. No.
Product Name
Price
L00440w
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
$179.00
L00440c
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
$179.00
L00440b
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
$179.00
L00439-1
Anti-His Affinity Resin - 1 ml
$399.00
L00439-5
Anti-His Affinity Resin - 5 ml
$1599.00
L00436
His Tag ELISA Detection Kit - 1 kit
$298.00
A01802-100
THETM His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$450.00
A01801-100
THETM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$450.00
A01800-100
THETM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$450.00
A01620
THETM His Tag Antibody [FITC], mAb, Mouse - 100 ug
$410.00
A00613
THETM His Tag Antibody [Biotin], mAb, Mouse - 100 ug
$410.00
A00612
THETM His Tag Antibody [HRP], mAb, Mouse - 100 ug
$410.00
A00205-40
His-tag antibody [Biotin], pAb, Rabbit - 40 ug
$99.00
A00186-100
THETM His Tag Antibody, mAb, Mouse - 100 ug
$375.00
A00174-40
His-tag Antibody, pAb, Rabbit - 40 ug
$99.00
 
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Yang M.,et al. The kinase Sgg modulates temporal development of macrochaetes in Drosophila by phosphorylation of Scute and Pannier.Development.2012Jan;139(2):325-34

Rosadini CV.,et al. A novel zinc binding system, ZevAB, is critical for survival of nontypeable Haemophilus influenzae in a murine lung infection model.Infect Immun.2011Aug;79(8):3366-3376

Duanpen Sandee and Walter L. Miller..,et al. High-Yield Expression of a Catalytically Active Membrane-Bound Protein: Human P450 Oxidoreductase.Endocrinology.2011Jul;152(7):2904 - 2908

Liu G.,et al. Reconstituted complexes of mycobacterial HSP70 and EBV LMP2A-derived peptides elicit peptide-specific cytotoxic T lymphocyte responses and anti-tumor immunity.Vaccine.2011Otc;29(43):7414-23

K Reifschneider-Wegner, et al. Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii. Int J Hydrogen Energy. 2014Jan.:289-294

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Cat. No. A00174
Size
Price
40 ug $99.00