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Catalog Products » Antibodies » Epitope Tag Antibodies » His-tag Antibody, pAb, Rabbit

His-tag Antibody, pAb, Rabbit

Synonyms His-tag Antibody, pAb, Rabbit;
Description His-tag Antibody, pAb, Rabbit recognizes His tagged proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen His epitope tag peptide HHHHHH conjugated to KLH
Purification Immunoaffinity chromatography

Subclass IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags. For N-terminal His-tagged protein detection, THETM Anti-His mAb (Cat. No. A00186) is recommended.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Cat. No. Product Name Quantity Price
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
Anti-His Affinity Resin - 1 ml
Anti-His Affinity Resin - 5 ml
His Tag ELISA Detection Kit - 1 kit
THE™ His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
THE™ His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
THE™ His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 ug
THE™ His Tag Antibody [Biotin], mAb, Mouse - 100 ug
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 ug
His-tag antibody [Biotin], pAb, Rabbit - 40 ug
THE™ His Tag Antibody, mAb, Mouse - 100 ug
His-tag Antibody, pAb, Rabbit - 40 ug
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Yang M.,et al. The kinase Sgg modulates temporal development of macrochaetes in Drosophila by phosphorylation of Scute and Pannier.Development.2012Jan;139(2):325-34

Raimunda D.,et al. A tetrahedral coordination of Zinc during transmembrane transport by P-type Zn 2 -ATPases.Biochim Biophys Acta.2012May;1818(5):1374-7

Sundlisaeter E.,et al. The alarmin IL-33 is a notch target in quiescent endothelial cells.Am J Pathol.2012Sep;181(3):1099-111

Tosca L.,et al. PLCγ, G-protein of the Gα q type and cADPr pathway are associated to trigger the fertilization Ca 2 signal in the sea urchin egg.Cell Calcium.2012Nov;52(5):388-96

Smit JJ.,et al. Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein–protein interaction.EMBO J.2013Feb;32(3):354-68

Wood GE.,et al. Persistence, Immune Response, and Antigenic Variation of Mycoplasma genitalium in an Experimentally Infected Pig-Tailed Macaque (Macaca nemestrina).Infect Immun.2013Jun;

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Cat. No. A00174
Size Price
40 ug $99.00