Catalog Products » Antibodies » Secondary Antibodies » Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb

Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb

Synonyms Donkey Anti-Goat IgG (H&L) [HRP]; Anti-Goat IgG (H&L) [HRP]
Description Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody is highly purified from donkey antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with goat IgG heavy and light chains.
Host Species Donkey
Antigen Species Goat
Conjugation Horseradish Peroxidase (HRP)
Immunogen Full-length goat IgG (purified)
Purification Immunoaffinity chromatography
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Subclass Donkey IgG
Concentration 1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
Reconstitution Reconstitute the lyophilized powder with 1 ml of deionized water (or equivalent) to antibody concentration of 1 mg/ml.
Species Reactivity Goat
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Donkey Anti-Goat IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:10,000-1:100,000
Western blot: 1:1,000-1:20,000
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Y Liu.,et al. Uncoupling Protein 2 Expression Affects Androgen Synthesis In Polycystic Ovary Syndrome.Endocrine.2013Jun;

Stengel A.,et al. Abdominal surgery inhibits circulating acyl ghrelin and ghrelin-O-acyltransferase levels in rats: role of the somatostatin receptor subtype 2.Am J Physiol Gastrointest Liver Physiol.2011Aug;301(2):G239 - G248

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Cat. No. A00178
1 mg $79.00