Catalog Products » Antibodies » Epitope Tag Antibodies » cGFP-tag Antibody, mAb, Mouse

cGFP-tag Antibody, mAb, Mouse

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Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein
Lane 4: Negative control
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

Lane 1: OFP transfecting 293 cell lysate
Lane 2: EGFP transfecting 293 cell lysate
Lane 3: 5 ng GFPuv protein
Lane 4: Negative 293 cell lysate
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)

Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein
Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
The Western blot was performed using One-Step Western^TM Basic Kit (GenScript, L00205)
with 4 µg of the antibody added to 4 ml WB solution.

Specificity analysis of THE^TM GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot
using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-mouse IgG.

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Overview

Synonyms	Mouse Anti-cGFP-tag mAb; Green Fluorescent Protein
Description	The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses.
Host Species	Mouse
Conjugation	Unconjugated
Immunogen	Purified recombinant cGFP fusion protein
Fusion Partner	Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification	Protein G affinity column
Key Features	GenScript cGFP-tag antibody has high sensitivity. GenScript cGFP-tag antibody can recognize various forms of GFP proteins including eGFP, OFP and GFPuv.

Properties

Subclass	Mouse IgG2b
Clone ID	5E5G11
Concentration	1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1 mg/ml.
Specificity	The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations
ELISA: 0.1-1.0 µg/ml
Western blot: 1.0-2.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00205C is recommended. 4 µg of this antibody is mixed with 4 ml of WB solution.
Other applications: user-optimized

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Yanjun Yang.,et al. A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana.New Phytol.2012Jan;193(1):81-95

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