cGFP-tag Antibody, mAb, Mouse
Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein
Lane 4: Negative control
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)
Lane 1: OFP transfecting 293 cell lysate
Lane 2: EGFP transfecting 293 cell lysate
Lane 3: 5 ng GFPuv protein
Lane 4: Negative 293 cell lysate
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)
Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein
Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
The Western blot was performed using One-Step WesternTM Basic Kit (GenScript, L00205)
with 4 µg of the antibody added to 4 ml WB solution.
Specificity analysis of THETM GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot
using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-mouse IgG.
| Synonyms | Mouse Anti-cGFP-tag mAb; Green Fluorescent Protein |
| Description | The coral green fluorescent protein (cGFP) from vector pRNATin-H1.2/Neo (GenScript, SD1223) is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. Mouse Anti-cGFP-tag Monoclonal Antibody can be used to detect cGFP fusion protein in Western blot analyses. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant cGFP fusion protein |
| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
| Purification | Protein G affinity column |
| Key Features |
| Subclass | Mouse IgG2b |
| Clone ID | 5E5G11 |
| Concentration | 1 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1 mg/ml. |
| Specificity | The antibody reacts with various forms of GFP including eGFP, OFP, and GFPuv. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| The investigator must determine the ideal working concentration for each specific application. We have not yet determined the suitability of this antibody for applications other than those listed below. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and length of incubations ELISA: 0.1-1.0 µg/ml Western blot: 1.0-2.0 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 4 µg of this antibody is mixed with 4 ml of WB solution. Other applications: user-optimized |
Lane 1-3: 100 ng, 25 ng, 10 ng GFP fusion protein
Lane 4: Negative control
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)
Lane 1: OFP transfecting 293 cell lysate
Lane 2: EGFP transfecting 293 cell lysate
Lane 3: 5 ng GFPuv protein
Lane 4: Negative 293 cell lysate
Primary antibody:
A. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
B. 1 µg/ml Mouse Anti-cGFP-tag Monoclonal Antibody (X Company)
Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:50,000)
Lane 1-3: 200 ng, 40 ng, 8 ng GFP fusion protein
Detection antibody: Mouse Anti-cGFP-tag Monoclonal Antibody (GenScript, A00185)
The Western blot was performed using One-Step WesternTM Basic Kit (GenScript, L00205)
with 4 µg of the antibody added to 4 ml WB solution.
Specificity analysis of THETM GFP Antibody, mAb, Mouse (GenScript, A00185) by Western blot
using a variety of variants of GFP protein such as eGFP, cGFP and AcGFP1.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-mouse IgG.
Y Liu.,et al. Characterization Of The Open Reading Frame 7A From Bombyx Mori Nucleopolyhedrovirus.Mol Biol Rep.2013Feb;
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W.-X. Liu.,et al. Inhibition effect produced by dominant negative mutant fusion protein PreS2‐TLM‐ScFv‐HBcDN on HBV replication in vitro.J Viral Hepat.2012Apr;19(4):295-300
Yanjun Yang.,et al. A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana.New Phytol.2012Jan;193(1):81-95
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| Cat. No. A00185 | ||
|---|---|---|
Size |
Price |
|
| 40 ug | $99.00 | |
| 100 ug | $199.00 | |
| 200 ug | $349.00 | |
