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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM His Tag Antibody, mAb, Mouse

THETM His Tag Antibody, mAb, Mouse

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
Lane 1: N-terminal His-tagged fusion protein
Lane 2: C-terminal His-tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of Multiple Tag Cell Lysate (GenScript, M0100) using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAbs (B: Competitor Q#1, 1 µg/ml; C: Competitor Q#2, 1 µg/ml) by Dot blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 0.1 µg/ml) with Mouse Anti-His mAb (B: Competitor A, 0.1 µg/ml) by Western blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAb (B: Competitor B, 1 µg/ml) by Western blot.
The assay was performed with cell lysates containing His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with His-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or His fusion gene transfected CHO cells using
THETM His Tag Antibody, mAb, Mouse (GenScript, A00186) (black and green, respectively).

The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
1. Positive control containg His fusion protein
2. Negative control – IP with isotype control antibody (A01007)
3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)

Synonyms THETM Anti-His mAb;
Description Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. However, since 6XHis-tag is poorly immunogenic, it needs to be conjugated to KLH or some other carrier as an immunogen. After hundreds of selection cycles, researchers at GenScript successfully isolated an antibody against His-tag.
THETM His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis-tags. This means that even if the 6xHis-tag is only partially exposed, it will still be recognized and bound by this antibody.
THETM His Tag mAb is produced from mice ascites and purified by protein A affinity column. This antibody recognizes native as well as denatured forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The product reacts with fusion proteins expressed in bacteria, insect cells, and mammalian cells. THETM His Tag mAb recognizes His tags placed at N-terminal, C-terminal, and internal regions of fusion proteins.
THETM His Tag mAb can be used in Western blot analyses, Dot blot analyses, ELISA, immunofluorescent staining, and flow cytometry of cultured cells.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide HHHHHH coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity THETM His Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western Blot: 0.1-0.2 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry (FACS): 1 µg/ml
TR-FRET assays: user-optimized
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of His-tagged fusion proteins using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
Lane 1: N-terminal His-tagged fusion protein
Lane 2: C-terminal His-tagged fusion protein
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of Multiple Tag Cell Lysate (GenScript, M0100) using THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml)
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAbs (B: Competitor Q#1, 1 µg/ml; C: Competitor Q#2, 1 µg/ml) by Dot blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 0.1 µg/ml) with Mouse Anti-His mAb (B: Competitor A, 0.1 µg/ml) by Western blot.
The assay was performed with His-tagged fusion protein.
The signal was developed with One-Step WesternTM Basic Kit (GenScript, L00205).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Comparison of THETM His Antibody, mAb, Mouse (A: GenScript, A00186, 1 µg/ml) with Mouse Anti-His mAb (B: Competitor B, 1 µg/ml) by Western blot.
The assay was performed with cell lysates containing His-tagged fusion protein.
The signal was developed with Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1#, 2#, 3# and 4# of THETM His Antibody, mAb, Mouse (GenScript, A00186, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with His-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Flow cytometric analysis of non-transfected or His fusion gene transfected CHO cells using
THETM His Tag Antibody, mAb, Mouse (GenScript, A00186) (black and green, respectively).

The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

THE<sup>TM</sup> His Tag Antibody, MAb, Mouse
THE<sup>TM</sup> His Tag Antibody, MAb, Mouse

Western blot analysis of immunoprecipitates from cell lysates containing His fusion protein using THETM His Antibody, mAb, Mouse (GenScript, A00186).
1. Positive control containg His fusion protein
2. Negative control – IP with isotype control antibody (A01007)
3. Immunoprecipitation with THETM His Tag Antibody, mAb, Mouse (A00186)


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Cat. No. A00186
Size
Price
100 ug $375.00
24 hour
 
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