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Support » Promotions » Product Promotions » Free Samples » THETM DYKDDDDK Tag Antibody, mAb, Mouse

THE™ DYKDDDDK Tag Antibody, mAb, Mouse

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Synonyms Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb;
Description The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THETM DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Note GenScript can offer this product per customer's reuqest including product size, buffer components,etc.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry: 1 µg/ml
Other applications: user-optimized

  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse
  • THE™ DYKDDDDK Tag Antibody, MAb, Mouse

    Flow cytometric analysis of non-transfected or Flag fusion gene transfected CHO cells using
    THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187) (black and yellow respectively). The signal was developed with FITC conjugated Goat Anti-Mouse IgG.

Cat. No. Product Name Quantity Price
THE™ DYKDDDDK Tag Antibody [Biotin], mAb, Mouse - 100 μg
THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse - 100 μg
Yan-Jhu Peng.,et al. A RING‐type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis.Plant J.2013Apr;74(2):310-27

Ivy KD.,et al. A Re-Evaluation of the Role of hCTR1, the Human High-Affinity Copper Transporter, in Platinum-Drug Entry into Human Cells.Mol Pharmacol.2013Jun;83(6):1237 - 1246

Maryon EB.,et al. Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1.Am J Physiol Cell Physiol.2013Apr;304(8):C768 - C779

Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;

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Cat. No. A00187S
Size Price
10 μg $0.00