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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM Flag Tag Antibody, mAb, Mouse

THETM DYKDDDDK Tag Antibody, mAb, Mouse

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Comparison of THETM DYKDDDDK Tag Antibody, mAb, Mouse (A: GenScript, A00187, 1 µg/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 µg/ml) by Western blot.
Lane 1 N-terminal DYKDDDDK-tagged fusion protein
Lane 2 Internal DYKDDDDK-tagged fusion protein
Lane 3 C-terminal DYKDDDDK-tagged fusion protein
Predicted Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
Observed Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Loading:
Lane 1-3 Multiple Tag (Purified) (GenScript, Cat.No.M0101, 400 ng, 80 ng,16 ng)
Lane 4. 293 cell lysate 10 μl
Primary antibody:
A. 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (GenScript, Cat. No. A00187, Lot No. A213902)
B. 1 μg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2)
Secondary antibody:
IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Western blot analysis of E.coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates.
1: E.coli lysate containing DYKDDDDK-tagged fusion protein
2: Immunoprecipitates of free E.coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.
3: Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

Synonyms Mouse anti DYKDDDDK-tag mAb; Mouse anti flag-tag mAb;
Description The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. THETM DYKDDDDK Tag Antibody, mAb, Mouse is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunoprecipitation: 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Flow cytometry: 1 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Comparison of THETM DYKDDDDK Tag Antibody, mAb, Mouse (A: GenScript, A00187, 1 µg/ml) with Mouse Anti- DYKDDDDK Tag mAb (B: Company S, clone M2, 1 µg/ml) by Western blot.
Lane 1 N-terminal DYKDDDDK-tagged fusion protein
Lane 2 Internal DYKDDDDK-tagged fusion protein
Lane 3 C-terminal DYKDDDDK-tagged fusion protein
Predicted Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
Observed Size:
N-terminal DYKDDDDK-tagged fusion protein 52 kD
Internal DYKDDDDK-tagged fusion protein 55 kD
C-terminal DYKDDDDK-tagged fusion protein 55 kD
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Loading:
Lane 1-3 Multiple Tag (Purified) (GenScript, Cat.No.M0101, 400 ng, 80 ng,16 ng)
Lane 4. 293 cell lysate 10 μl
Primary antibody:
A. 1 μg/ml THETM Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (GenScript, Cat. No. A00187, Lot No. A213902)
B. 1 μg/ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Company S, clone M2)
Secondary antibody:
IRDyeTM800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000,610-132-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Western blot analysis of E.coli lysate containing DYKDDDDK-tagged fusion protein and its immunoprecipitates.
1: E.coli lysate containing DYKDDDDK-tagged fusion protein
2: Immunoprecipitates of free E.coli lysate incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.
3: Immunoprecipitates of E.coli lysate containing DYKDDDDK-tagged fusion protein incubated with DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 2 µg/ml) and Protein A.

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with C-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

293 cells transfected with N-terminal DYKDDDDK tag protein
Primary antibody: 1 μg/ml THETM Anti-DYKDDDDK-tag Monoclonal Antibody (Mouse) (GenScript, A00187)
Secondary antibody: 2 μg/ml Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (Rockland, 610-102-121)

 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse
 THE<sup>TM</sup> DYKDDDDK Tag Antibody, MAb, Mouse

Consistency analysis of Batch 1# and 2# of THETM DYKDDDDK Tag Antibody, mAb, Mouse (GenScript, A00187, 1 µg/ml) by Western blot, showing that signal remains consistent from Lot to Lot.
The assay was performed with DYKDDDDK-tagged fusion protein.
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Mouse IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00170-40 DYKDDDDK-tag Antibody, pAb, Rabbit
40 ug
$99.00
A01428-100 THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse
100 ug
$149.00
A01429-100 THETM DYKDDDDK Tag Antibody [Biotin], mAb, Mouse
100 ug
$149.00
A01632 THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse
100 ug
$149.00
L00425 Anti-DYKDDDDK IP Resin
1 ml
$199.00
L00432-1 Anti-DYKDDDDK G1 Affinity Resin
1 ml
$281.00
L00432-25 Anti-DYKDDDDK G1 Affinity Resin
25 ml
$2591.00
L00432-5 Anti-DYKDDDDK G1 Affinity Resin
5 ml
$663.00
L00455W DYKDDDDK Tag Antibody Plate (White, 96-well)
5 plates
$229.00
L00455B DYKDDDDK Tag Antibody Plate (Black, 96-well)
5 plates
$229.00
A01810-100 THETM DYKDDDDK Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$167.00
A01811-100 THETM DYKDDDDK Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$167.00
 
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Ivy KD.,et al. A Re-Evaluation of the Role of hCTR1, the Human High-Affinity Copper Transporter, in Platinum-Drug Entry into Human Cells.Mol Pharmacol.2013Jun;83(6):1237 - 1246\x09\x09\x09

Yan-Jhu Peng.,et al. A RING‐type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis.Plant J.2013Apr;74(2):310-27

Reuben A, et al. The WT hemochromatosis protein HFE inhibits CD8 T‐lymphocyte activation. Eur J Immunol. 2014Mar.;

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Cat. No. A00187
Size
Price
200 ug $256.00
100 ug $139.00
24 hour
 
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