|Synonyms||Mouse Anti-Myc-tag mAb;|
|Description||A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH was used as immunogen. Myc-tag provides a method to localize gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins.|
|Immunogen||A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH|
|Fusion Partner||Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.|
|Purification||Protein G affinity column|
|Concentration||100 μg/100 μl in PBS, pH7.4, containing 0.02% sodium azide|
|Storage||Store at 4°C short term. Aliquot and store at -20°C or -8°C. Avoid repeated freeze/thaw cycles.|
|Note||Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.|
|Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1 µg/ml
Western blot: 1.0 µg/ml (5% BSA is recommended as blocking reagent)
Other applications: user-optimized
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