|Synonyms||MBP-tag Monoclonal Antibody;|
|Description||Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP tagged protein is purified with an amylose column. The MBP tagged protein can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein. MBP-tag Antibody, mAb, Mouse is suitable for detecting fusion proteins that contain a MBP-tag.
GenScript MBP-tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant MBP fusion protein.
|Immunogen||Purified recombinant MBP fusion protein|
|Fusion Partner||Spleen cells are fused to SP2/0-Ag14 mouse myeloma cells.|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||GenScript MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.5-1 µg/ml
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
|Cat. No. A00190|