×
Catalog Products » Antibodies » Epitope Tag Antibodies » MBP-tag Antibody, mAb, Mouse

MBP-tag Antibody, mAb, Mouse

Synonyms MBP-tag Monoclonal Antibody;
Description Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP tagged protein is purified with an amylose column. The MBP tagged protein can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein. MBP-tag Antibody, mAb, Mouse is suitable for detecting fusion proteins that contain a MBP-tag.
GenScript MBP-tag Antibody, mAb, Mouse is produced from the hybridoma resulting from fusion of Sp2/0 myeloma and lymphocytes obtained from mouse immunized with purified recombinant MBP fusion protein.
Host Species Mouse
Conjugation Unconjugated
Immunogen Purified recombinant MBP fusion protein
Fusion Partner Spleen cells are fused to SP2/0-Ag14 mouse myeloma cells.
Purification Protein A affinity column

Subclass Mouse IgG1
Clone ID 5B10B11
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript MBP-tag Antibody, mAb, Mouse specifically reacts with fusion proteins containing MBP epitope tags.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.1-1.0 µg/ml
Western blot: 0.5-1 µg/ml
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IP Protocol(pdf)
IHC Protocol(pdf)

MBP-tag Antibody, MAb, Mouse
MBP-tag Antibody, MAb, Mouse

Western blot analysis of MBP-tagged fusion protein using MBP-tag Antibody, mAb, Mouse (GenScript, A00190, 1 µg/ml)
The signal was developed with IRDye™ 800 Conjugated Goat Anti-Mouse IgG.
Predicted Size: 58 kD
Observed Size: 58 kD


Lee SY., et al. The Influence of T Cell Ig Mucin-3 Signaling on Central Nervous System Autoimmune Disease Is Determined by the Effector Function of the Pathogenic T Cells. J Immunol. 2013 May;190(10):4991-9.

Wang L.,et al. A novel C-type lectin from crab Eriocheir sinensis functions as pattern recognition receptor enhancing cellular encapsulation.Fish Shellfish Immunol.2013Jan;S1050-4648(12):00477-9

You can visit "Citations Database" for more citations.







For more documents, please visit "Technical Support".

Cat. No. A00190
Size
Price
100 ug $199.00
 
*
*
*
*