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Catalog Products » Antibodies » Primary Antibodies » GPCR Antibodies » GAPDH Antibody, pAb, Goat

GAPDH Antibody, pAb, Goat

GAPDH Antibody, PAb, Goat

Western blot analysis of cell lysates using GAPDH Antibody, pAb, Goat (GenScript, A00191, 0.5 µg/ml)
The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 36 kD
Observed Size: 36 kD

GAPDH Antibody, PAb, Goat

Fluorescent Western blots for the detection of GAPDH using the One-Step WesternTM Fluorescent Kit (GenScript, L00397).
Lane 1-8: 5.0, 2.5, 1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 µg of HeLa cell lysate.

GAPDH Antibody, PAb, Goat

Western blot analysis of cell lysates using GAPDH Antibody, pAb, Goat (GenScript, A00191, 0.1 µg/ml)
The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 36 kD
Observed Size: 36 kD

Synonyms Anti-GAPDH;
Description Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Though expressed differently in different tissues, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH is often used as a control in experiments quantifying specific changes in expression levels of other targets. Recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular processes, such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. GAPDH may also play a role in neurodegenerative pathologies such as Huntingtons and Alzheimers disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. GenScript Goat Anti-GAPDH Polyclonal Antibody is highly purified from goat antiserum by immunoaffinity chromatography.
Host Species Goat
Conjugation Unconjugated
Immunogen Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle
Purification Immunoaffinity chromatography

Subclass Goat IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript Goat Anti-GAPDH Polyclonal Antibody specifically reacts with GAPDH from human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish, and E. coli cell and tissue lysates. It has not yet been tested with other species.
Species Reactivity Human, mouse, rat, goat, rabbit, chicken, hamster, bovine, swine, fish and E. coli
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

  • GAPDH Antibody, PAb, Goat
  • GAPDH Antibody, PAb, Goat

    Western blot analysis of cell lysates using GAPDH Antibody, pAb, Goat (GenScript, A00191, 0.5 µg/ml)
    The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
    Predicted Size: 36 kD
    Observed Size: 36 kD

  • GAPDH Antibody, PAb, Goat
  • GAPDH Antibody, PAb, Goat

    Fluorescent Western blots for the detection of GAPDH using the One-Step WesternTM Fluorescent Kit (GenScript, L00397).
    Lane 1-8: 5.0, 2.5, 1.25, 0.62, 0.31, 0.16, 0.08 and 0.04 µg of HeLa cell lysate.

  • GAPDH Antibody, PAb, Goat
  • GAPDH Antibody, PAb, Goat

    Western blot analysis of cell lysates using GAPDH Antibody, pAb, Goat (GenScript, A00191, 0.1 µg/ml)
    The signal was developed with Donkey Anti-Goat IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00178, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
    Predicted Size: 36 kD
    Observed Size: 36 kD


Cat. No.
Product Name
Price
A00915-40
GAPDH Antibody [Biotin], pAb, Goat - 40 ug
$99.00
A00192-40
GAPDH Antibody [HRP], pAb, Goat - 40 ug
$99.00
A00178
Donkey Anti-Goat IgG Antibody (H&L) [HRP], pAb - 1 mg
$79.00
 
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Cat. No. A00191
Size
Price
40 ug $99.00