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Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » His Tagged Protein Detection & Purification » THETM His Tag Antibody [HRP], mAb, Mouse

THETM His Tag Antibody [HRP], mAb, Mouse

Synonyms His-tag Antibody [HRP],mAb, Mouse;
Description GenScript THETM His Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-His Monoclonal Antibody (Mouse) (A00186) purified from mice ascites by protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins or His by various immunoassays.
Host Species Mouse
Conjugation Horseradish Peroxidase (HRP)
Immunogen A synthetic peptide HHHHHH coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
Specificity THETM His Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.5 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  • THE<sup>TM</sup> His Tag Antibody [HRP], MAb, Mouse
  • THE<sup>TM</sup> His Tag Antibody [HRP], MAb, Mouse

    Western blot analysis of His-tagged protein using THETM His Tag Antibody [HRP], mAb, Mouse (A00612, 1 µg/ml) The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)


Which resin yields 40-60 mg His-tagged protein
per ml settled resin?

Find answer here...

GenScript’s High Affinity Ni-Charged resin

Cat. No.
Product Name
Price
A00174-40
His-tag Antibody, pAb, Rabbit - 40 ug
$99.00
A00186-100
THETM His Tag Antibody, mAb, Mouse - 100 ug
$375.00
A00205-40
His-tag antibody [Biotin], pAb, Rabbit - 40 ug
$99.00
A00613
THETM His Tag Antibody [Biotin], mAb, Mouse - 100 ug
$410.00
A01620
THETM His Tag Antibody [FITC], mAb, Mouse - 100 ug
$410.00
L00436
His Tag ELISA Detection Kit - 1 kit
$298.00
L00439-1
Anti-His Affinity Resin - 1 ml
$399.00
L00439-5
Anti-His Affinity Resin - 5 ml
$1599.00
L00440c
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
$179.00
L00440w
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
$179.00
L00440b
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
$179.00
A01800-100
THETM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$450.00
A01801-100
THETM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$450.00
A01802-100
THETM His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$450.00
 
Melnikow E.,et al. A Potential Role for the Interaction of Wolbachia Surface Proteins with the Brugia malayi Glycolytic Enzymes and Cytoskeleton in Maintenance of Endosymbiosis.PLoS Negl Trop Dis.2013Apr;7(4):e2151

J Jarmander.,et al. A Dual Tag System For Facilitated Detection Of Surface Expressed Proteins In Escherichia Coli.Microb Cell Fact.2012Sep;11(1):118

G Isik, et al. Enhanced Immunogenicity of HIV-1 Envelope gp140 Proteins Fused to APRIL. PLoS One. 2014Sep.;9(9):e107683

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For more documents, please visit "Technical Support".

Cat. No. A00612
Size
Price
100 ug $410.00
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