Catalog Products » Protein Isolation and Analysis » Protein Detection and Immunoassay » His Tagged Protein Detection & Purification » THETM His Tag Antibody [Biotin], mAb, Mouse

THETM His Tag Antibody [Biotin], mAb, Mouse

Synonyms THETM His Tag Antibody [Biotin], mAb, Mouse;
Description GenScript THETM His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THETM His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of His in fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen A synthetic peptide (HHHHHH) coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  •  THE<sup>TM</sup> His Tag Antibody [Biotin], MAb, Mouse
  •  THE<sup>TM</sup> His Tag Antibody [Biotin], MAb, Mouse

    Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml)
    The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 52 KD
    Observed Size: 52 KD

Cat. No.
Product Name
His-tag Antibody, pAb, Rabbit - 40 ug
THETM His Tag Antibody, mAb, Mouse - 100 ug
His-tag antibody [Biotin], pAb, Rabbit - 40 ug
THETM His Tag Antibody [FITC], mAb, Mouse - 100 ug
His Tag ELISA Detection Kit - 1 kit
Anti-His Affinity Resin - 1 ml
Anti-His Affinity Resin - 5 ml
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
THETM His Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
THETM His Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
THETM His Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
Fiona Wegman.,et al. Gene delivery of bone morphogenetic protein‐2 plasmid DNA promotes bone formation in a large animal model.J Tissue Eng Regen Med.2012Aug;

Takayuki Ota.,et al. Anti-Hiv B Cell Lines As Candidate Vaccine Biosensors.J Immunol.2012Nov;189(10):4816 - 4824

V Hernandez-Gordillo, et al. Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds. Biomaterials. 2014Aug.;35(26):7363-73

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For more documents, please visit "Technical Support".

Cat. No. A00613
100 ug $410.00