• English
  • Sign In
  • Contact Us
Catalog Products » Antibodies » Epitope Tag Antibodies » THETM His Tag Antibody [Biotin], mAb, Mouse

THE™ His Tag Antibody [Biotin], mAb, Mouse

Synonyms THETM His Tag Antibody [Biotin], mAb, Mouse;
Description GenScript THETM His Tag Antibody [Biotin], mAb, mouse is the biotin conjugated THETM His Tag Antibody, mAb, mouse (A00186) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of His in fusion proteins.
Host Species Mouse
Conjugation Biotin
Immunogen A synthetic peptide (HHHHHH) coupled to KLH
Purification Protein A affinity column

Subclass IgG1, k
Clone ID 6G2A9
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity GenScript THETM His Tag Antibody [Biotin], mAb, Mouse specificly recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.02-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other applications: user-optimized

  • THE™ His Tag Antibody [Biotin], MAb, Mouse
  • THE™ His Tag Antibody [Biotin], MAb, Mouse

    Western blot analysis of His tagged fusion protein (Genscript, M0100) using THETM His Tag Antibody [Biotin], mAb, mouse (GenScript, A00613, 1 µg/ml)
    The signal was developed with Streptavidin-HRP (GenScript, M00091, 1:10,000) and and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 52 KD
    Observed Size: 52 KD

Cat. No. Product Name Quantity Price
Streptavidin-HRP - 1 mg
His Tag Antibody Plate (white, 96 wells, nonremovable) - 5 plates
His Tag Antibody Plate (clear, 8x12 strips) - 5 plates
His Tag Antibody Plate (black, 96 wells, nonremovable) - 5 plates
Anti-His Affinity Resin - 1 ml
Anti-His Affinity Resin - 5 ml
His Tag ELISA Detection Kit - 1 kit
THE™ His Tag Antibody [iFluor 647], mAb, Mouse - 100 μg
THE™ His Tag Antibody [iFluor 555], mAb, Mouse - 100 μg
THE™ His Tag Antibody [iFluor 488], mAb, Mouse - 100 μg
THE™ His Tag Antibody [FITC], mAb, Mouse - 100 μg
THE™ His Tag Antibody [HRP], mAb, Mouse - 100 μg
His-tag antibody [Biotin], pAb, Rabbit - 40 μg
THE™ His Tag Antibody, mAb, Mouse - 100 μg
His-tag Antibody, pAb, Rabbit - 40 μg
V Hernandez-Gordillo, et al. Mimicking the extracellular matrix with functionalized, metal-assembled collagen peptide scaffolds. Biomaterials. 2014Aug.;35(26):7363-73

Takayuki Ota.,et al. Anti-Hiv B Cell Lines As Candidate Vaccine Biosensors.J Immunol.2012Nov;189(10):4816 - 4824

Fiona Wegman.,et al. Gene delivery of bone morphogenetic protein‐2 plasmid DNA promotes bone formation in a large animal model.J Tissue Eng Regen Med.2012Aug;

You can visit "Citations Database" for more citations.

For more documents, please visit "Technical Support".

Cat. No. A00613
Size Price
100 μg $410.00