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Catalog Products » Antibodies » Primary Antibodies » GPCR Antibodies » cAMP Antibody, pAb, Rabbit

cAMP Antibody, pAb, Rabbit

Synonyms Rabbit Anti cAMP pAb;
Description Adenosine 3', 5'-cyclic monophosphate (cyclic AMP; cAMP) is one of the most important intracellular secondary messengers for transduction events. cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune, and other functions and actions. A number of hormones, such as ACTH, TSH, FSH, and LH, are known to activate cAMP through the action of the enzyme adenylate cyclase, which converts ATP to cAMP. There remains considerable interest in the measurement of intracellular cAMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states. Due to the involvement of cAMP in amplifying the response of ligand binding, the second messenger cAMP has been largely employed to monitor the activation of GPCR to facilitate therapeutic drug discovery.
GenScript Rabbit Anti-cAMP Polyclonal Antibody is developed in rabbit using 3', 5'-cyclic AMP-8-KLH as the immunogen.
Host Species Rabbit
Conjugation Unconjugated
Immunogen 3', 5'-cyclic AMP-8-KLH
Purification Antiserum

Subclass Rabbit IgG
Specificity The specificity of antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding. The cross-reactivity data obtained in competitive ELISA system is as follows:
Compound % Cross-reactivity
cAMP 100
AMP 0.0140
cGMP 0.0290
GMP 0.0223
ADP 0.0069
GDP 0.0011
ATP <0.0001
GTP <0.0001
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:4,000-1:40,000
Sensitivity: Sensitivity is defined as the 90% intercept of a B/B0 standard curve. In competitive ELISA system the sensitivity has been found to be 1-50 fmol/well.

  • CAMP Antibody, PAb, Rabbit
  • CAMP Antibody, PAb, Rabbit

    Cross-reactivity analysis of cAMP Antibody, pAb,
    Rabbit (GenScript, A00614) by competitive ELISA.


Cat. No.
Product Name
Price
M01059
cAMP-HRP - 200 μl
$105.00
A01509-100
THETM cAMP Antibody, mAb, Mouse - 100 ug
$330.00
A01509-40
THETM cAMP Antibody, mAb, Mouse - 40 ug
$158.00
A01509-200
THETM cAMP Antibody, mAb, Mouse - 200 ug
$594.00
 


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Cat. No. A00614
Size
Price
40 μl $99.00