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Catalog Products » Antibodies » Epitope Tag Antibodies » V5-tag Antibody, pAb, Rabbit

V5-tag Antibody, pAb, Rabbit

V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-V5-tag pAb; Anti-V5-tag
Description The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Host Species Rabbit
Conjugation Unconjugated
Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml,lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

V5-tag Antibody, PAb, Rabbit
V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

V5-tag Antibody, PAb, Rabbit
V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

V5-tag Antibody, PAb, Rabbit
V5-tag Antibody, PAb, Rabbit

Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00877-40 V5-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
A01724-40 THETM V5 Tag Antibody, mAb, Mouse
40 ug
$119.00
A01724-100 THETM V5 Tag Antibody, mAb, Mouse
100 ug
$219.00
A01724-200 THETM V5 Tag Antibody, mAb, Mouse
200 ug
$395.00
A01733-100 THETM V5 Tag Antibody [HRP], mAb, Mouse
100 ug
$239.00
A01735-100 THETM V5 Tag Antibody [Biotin], mAb, Mouse
100 ug
$239.00
A00877-100 V5-tag Antibody [HRP], pAb, Rabbit
100 ug
$199.00
A01803-100 THETM V5 Tag Antibody [iFluor 488], mAb, Mouse
100 ug
$263.00
A01804-100 THETM V5 Tag Antibody [iFluor 555], mAb, Mouse
100 ug
$263.00
A01805-100 THETM V5 Tag Antibody [iFluor 647], mAb, Mouse
100 ug
$263.00
 
Afrazi A.,et al. Intracellular Heat Shock Protein-70 Negatively Regulates TLR4 Signaling in the Newborn Intestinal Epithelium.J Immunol.2012May;

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Cat. No. A00623
Size
Price
100 ug $199.00
24 hour
 
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