V5-tag Antibody, pAb, Rabbit

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Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step Western^TM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

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Overview

Synonyms	Rabbit Anti-V5-tag pAb; Anti-V5-tag
Description	The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml,lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity	GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Examples

Related Products

Cat. No.	Product Name	Price
A01805-100	THE^TM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug	$263.00
A01804-100	THE^TM V5 Tag Antibody [iFluor 555], mAb, Mouse - 100 ug	$263.00
A01803-100	THE^TM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug	$263.00
A01735-100	THE^TM V5 Tag Antibody [Biotin], mAb, Mouse - 100 ug	$239.00
A01733-100	THE^TM V5 Tag Antibody [HRP], mAb, Mouse - 100 ug	$239.00
A01724-40	THE^TM V5 Tag Antibody, mAb, Mouse - 40 ug	$119.00
A01724-100	THE^TM V5 Tag Antibody, mAb, Mouse - 100 ug	$219.00
A01724-200	THE^TM V5 Tag Antibody, mAb, Mouse - 200 ug	$395.00
A00877-40	V5-tag Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
A00877-100	V5-tag Antibody [HRP], pAb, Rabbit - 100 ug	$199.00
A00098	Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb - 1 mg	$79.00

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Afrazi A.,et al. Intracellular Heat Shock Protein-70 Negatively Regulates TLR4 Signaling in the Newborn Intestinal Epithelium.J Immunol.2012May;

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