V5-tag Antibody, pAb, Rabbit
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Synonyms | Rabbit Anti-V5-tag pAb; Anti-V5-tag |
| Description | The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope. |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Immunogen | V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH |
| Purification | Immunoaffinity chromatography |
| Subclass | Rabbit IgG |
| Concentration | 0.5 mg/ml,lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml. |
| Specificity | GenScript Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized |
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5-tagged fusion protein using V5-tag Antibody, pAb, Rabbit (GenScript, A00623, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204)
Predicted Size: 52 kD
Observed Size: 52 kD
Western blot analysis of V5 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-V5-tag Polyclonal Antibody (GenScript, A00623, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01805-100 |
THE™ V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug |
$263.00 |
|
A01804-100 |
THE™ V5 Tag Antibody [iFluor 555], mAb, Mouse - 100 ug |
$263.00 |
|
A01803-100 |
THE™ V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug |
$263.00 |
|
A01735-100 |
THE™ V5 Tag Antibody [Biotin], mAb, Mouse - 100 ug |
$239.00 |
|
A01733-100 |
THE™ V5 Tag Antibody [HRP], mAb, Mouse - 100 ug |
$239.00 |
|
A01724-100 |
THE™ V5 Tag Antibody, mAb, Mouse - 100 ug |
$219.00 |
|
A01724-200 |
THE™ V5 Tag Antibody, mAb, Mouse - 200 ug |
$395.00 |
|
A00877-40 |
V5-tag Antibody [HRP], pAb, Rabbit - 40 ug |
$99.00 |
|
A00098 |
Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb - 1 mg |
$79.00 |
Afrazi A.,et al. Intracellular Heat Shock Protein-70 Negatively Regulates TLR4 Signaling in the Newborn Intestinal Epithelium.J Immunol.2012May;
You can visit "Citations Database" for more citations.
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A00623 | ||
|---|---|---|
| Size | Price | |
| 40 ug | $99.00 | |
