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Catalog Products » Antibodies » Epitope Tag Antibodies » HSV-tag Antibody, pAb, Rabbit

HSV-tag Antibody, pAb, Rabbit

HSV-tag Antibody, PAb, Rabbit

Western blot analysis of HSV-tagged fusion protein using HSV-tag Antibody, pAb, Rabbit (GenScript, A00624, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

HSV-tag Antibody, PAb, Rabbit

Western blot analysis of HSV tagged fusion proteins expressed in E. coli cell lysate using HSV-tag Antibody (GenScript, A00624, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-HSV-tag pAb; Anti-HSV-tag
Description GenScript Rabbit Anti-HSV-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen HSV epitope tag peptide QPELAPEDPED conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity This Antibody recognizes HSV tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

HSV-tag Antibody, PAb, Rabbit
HSV-tag Antibody, PAb, Rabbit

Western blot analysis of HSV-tagged fusion protein using HSV-tag Antibody, pAb, Rabbit (GenScript, A00624, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

HSV-tag Antibody, PAb, Rabbit
HSV-tag Antibody, PAb, Rabbit

Western blot analysis of HSV tagged fusion proteins expressed in E. coli cell lysate using HSV-tag Antibody (GenScript, A00624, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Anania VG.,et al. A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway.Int J Proteomics.2013Feb;

Uribe R.,et al. A functional study of nucleocytoplasmic transport signals of the EhNCABP166 protein from Entamoeba histolytica.Parasitology.2012Nov;139(12):1697-710

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Cat. No. A00624
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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