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HSV-tag Antibody, pAb, Rabbit

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Western blot analysis of HSV-tagged fusion protein using HSV-tag Antibody, pAb, Rabbit (GenScript, A00624, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

Western blot analysis of HSV tagged fusion proteins expressed in E. coli cell lysate using HSV-tag Antibody (GenScript, A00624, 1 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

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Overview

Synonyms	Rabbit Anti-HSV-tag pAb; Anti-HSV-tag
Description	GenScript Rabbit Anti-HSV-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	HSV epitope tag peptide QPELAPEDPED conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	This Antibody recognizes HSV tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Anania VG.,et al. A Novel Peptide-Based SILAC Method to Identify the Posttranslational Modifications Provides Evidence for Unconventional Ubiquitination in the ER-Associated Degradation Pathway.Int J Proteomics.2013Feb;

Uribe R.,et al. A functional study of nucleocytoplasmic transport signals of the EhNCABP166 protein from Entamoeba histolytica.Parasitology.2012Nov;139(12):1697-710

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