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Catalog Products » Antibodies » Epitope Tag Antibodies » S-tag Antibody, pAb, Rabbit

S-tag Antibody, pAb, Rabbit

S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion protein using S-tag Antibody, pAb, Rabbit (GenScript, A00625, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion protein using S-tag Antibody, pAb, Rabbit (GenScript, A00625, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-S-tag Polyclonal Antibody (GenScript, A00625, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-S-tag pAb; Anti-S-tag
Description S-tag is derived from pancreatic ribonuclease A (RNase A). With its abundance of charged and polar residues, the tag could improve solubility of recombinant proteins. It can be fused at the N- or C-terminus of target protein. S-tag Antibody is a useful tool in detection of recombinant proteins containing the S-tag.
Host Species Rabbit
Conjugation Unconjugated
Immunogen S epitope tag peptide KETAAAKFERQHMDS conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-S-tag Polyclonal Antibody specifically recognizes S tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot:
0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit:
For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

S-tag Antibody, PAb, Rabbit
S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion protein using S-tag Antibody, pAb, Rabbit (GenScript, A00625, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 52 kD
Observed Size: 52 kD

S-tag Antibody, PAb, Rabbit
S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion protein using S-tag Antibody, pAb, Rabbit (GenScript, A00625, 1 µg/ml)
The signal was developed with One-Step WesternTM Basic Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

S-tag Antibody, PAb, Rabbit
S-tag Antibody, PAb, Rabbit

Western blot analysis of S-tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-S-tag Polyclonal Antibody (GenScript, A00625, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A00625
Size
Price
100 ug $199.00
 
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