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Catalog Products » Antibodies » THE™ Elite Antibodies » THE™ HA-tag mAb » NWSHPQFEK Antibody, pAb, Rabbit

NWSHPQFEK Antibody, pAb, Rabbit

NWSHPQFEK Antibody, PAb, Rabbit

Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 48 kD
Observed Size: 48 kD

NWSHPQFEK Antibody, PAb, Rabbit

Western blot analysis of C-terminal NWSHPQFEK tagged fusion proteins expressed in E. coli cell lysate using NWSHPQFEK-tag Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-NWSHPQFEK Polyclonal Antibody; Anti-NWSHPQFEK
Description Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems. The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered.
GenScript Rabbit Anti-NWSHPQFEK Polyclonal Antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.
Host Species Rabbit
Conjugation Unconjugated
Immunogen This epitope tag peptide NWSHPQFEK conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Specificity This antibody recognizes NWSHPQFEK tagged fusion protein.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

NWSHPQFEK Antibody, PAb, Rabbit
NWSHPQFEK Antibody, PAb, Rabbit

Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 48 kD
Observed Size: 48 kD

NWSHPQFEK Antibody, PAb, Rabbit
NWSHPQFEK Antibody, PAb, Rabbit

Western blot analysis of C-terminal NWSHPQFEK tagged fusion proteins expressed in E. coli cell lysate using NWSHPQFEK-tag Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00626-40 NWSHPQFEK Antibody, pAb, Rabbit
40 ug
$99.00
A01732-100 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
100 ug
$319.00
A01732-200 THETM NWSHPQFEK Tag Antibody,mAb,Mouse
200 ug
$579.00
A01736-100 THETM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse
100 ug
$349.00
A01737-100 THETM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse
100 ug
$349.00
A01742-40 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
40 ug
$154.00
A01742-100 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
100 ug
$349.00
A01742-200 THETM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse
200 ug
$637.00
 
Miranda HV.,et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins by a UbaA-dependent mechanism.Mol Cell Proteomics.2013Otc;

Turner L.,et al. Severe malaria is associated with parasite binding to endothelial protein C receptor.Nature.2013Jun;

Avril M.,et al. DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells.PLoS Pathog.2013Jun;9(6):e1003430

John P. Welsh.,et al. Cell‐free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens.Biotechnol Bioeng.2012Dec;109(12):2962-9

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Cat. No. A00626
Size
Price
40 ug $99.00
24 hour
 
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