NWSHPQFEK Antibody, pAb, Rabbit Antibody

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Overview

Synonyms	Rabbit Anti-NWSHPQFEK Polyclonal Antibody; Anti-NWSHPQFEK
Description	Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems. The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. GenScript Rabbit Anti-NWSHPQFEK Polyclonal Antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	This epitope tag peptide NWSHPQFEK conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml.
Specificity	This antibody recognizes NWSHPQFEK tagged fusion protein.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Examples

Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensor^TM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 48 kD
Observed Size: 48 kD

Western blot analysis of C-terminal NWSHPQFEK tagged fusion proteins expressed in E. coli cell lysate using NWSHPQFEK-tag Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with IRDye^TM 800 Conjugated Goat Anti-Rabbit IgG.

Related Products

Cat. No.	Product Name	Price
A01742-40	THE^TM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 40 ug	$154.00
A01742-100	THE^TM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 100 ug	$349.00
A01742-200	THE^TM NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 200 ug	$637.00
A01737-100	THE^TM NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse - 100 ug	$349.00
A01736-100	THE^TM NWSHPQFEK Tag Antibody [FITC], mAb, Mouse - 100 ug	$349.00
A01732-100	THE^TM NWSHPQFEK Tag Antibody,mAb,Mouse - 100 ug	$319.00
A01732-200	THE^TM NWSHPQFEK Tag Antibody,mAb,Mouse - 200 ug	$579.00
A00875-40	NWSHPQFEK Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
A00875-100	NWSHPQFEK Antibody [HRP], pAb, Rabbit - 100 ug	$199.00
A00626-40	NWSHPQFEK Antibody, pAb, Rabbit - 40 ug	$99.00

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Miranda HV.,et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins by a UbaA-dependent mechanism.Mol Cell Proteomics.2013Otc;

Turner L.,et al. Severe malaria is associated with parasite binding to endothelial protein C receptor.Nature.2013Jun;

Avril M.,et al. DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells.PLoS Pathog.2013Jun;9(6):e1003430

John P. Welsh.,et al. Cellâ€free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens.Biotechnol Bioeng.2012Dec;109(12):2962-9

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