NWSHPQFEK Antibody, pAb, Rabbit
Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 48 kD
Observed Size: 48 kD
Western blot analysis of C-terminal NWSHPQFEK tagged fusion proteins expressed in E. coli cell lysate using NWSHPQFEK-tag Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Synonyms | Rabbit Anti-NWSHPQFEK Polyclonal Antibody; Anti-NWSHPQFEK |
| Description | Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems. The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. GenScript Rabbit Anti-NWSHPQFEK Polyclonal Antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH. |
| Host Species | Rabbit |
| Conjugation | Unconjugated |
| Immunogen | This epitope tag peptide NWSHPQFEK conjugated to KLH |
| Purification | Immunoaffinity chromatography |
| Subclass | Rabbit IgG |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to antibody concentration of 0.5 mg/ml. |
| Specificity | This antibody recognizes NWSHPQFEK tagged fusion protein. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Other applications: user-optimized |
Western blot analysis of NWSHPQFEK-tagged fusion protein using NWSHPQFEK Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221)
Predicted Size: 48 kD
Observed Size: 48 kD
Western blot analysis of C-terminal NWSHPQFEK tagged fusion proteins expressed in E. coli cell lysate using NWSHPQFEK-tag Antibody, pAb, Rabbit (GenScript, A00626, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A01742-100 |
THE™ NWSHPQFEK Tag Antibody [HRP], mAb, Mouse - 100 ug |
$349.00 |
|
A01737-100 |
THE™ NWSHPQFEK Tag Antibody [Biotin], mAb, Mouse - 100 ug |
$349.00 |
|
A01736-100 |
THE™ NWSHPQFEK Tag Antibody [FITC], mAb, Mouse - 100 ug |
$349.00 |
|
A01732-100 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 100 ug |
$319.00 |
|
A01732-200 |
THE™ NWSHPQFEK Tag Antibody,mAb,Mouse - 200 ug |
$579.00 |
|
A00875-40 |
NWSHPQFEK Antibody [HRP], pAb, Rabbit - 40 ug |
$99.00 |
|
A00626-40 |
NWSHPQFEK Antibody, pAb, Rabbit - 40 ug |
$99.00 |
John P. Welsh.,et al. Cellâ€free production of trimeric influenza hemagglutinin head domain proteins as vaccine antigens.Biotechnol Bioeng.2012Dec;109(12):2962-9
Avril M.,et al. DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells.PLoS Pathog.2013Jun;9(6):e1003430
Turner L.,et al. Severe malaria is associated with parasite binding to endothelial protein C receptor.Nature.2013Jun;
Miranda HV.,et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins by a UbaA-dependent mechanism.Mol Cell Proteomics.2013Otc;
You can visit "Citations Database" for more citations.
Avril M.,et al. DC8 and DC13 var Genes Associated with Severe Malaria Bind Avidly to Diverse Endothelial Cells.PLoS Pathog.2013Jun;9(6):e1003430
Turner L.,et al. Severe malaria is associated with parasite binding to endothelial protein C receptor.Nature.2013Jun;
Miranda HV.,et al. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins by a UbaA-dependent mechanism.Mol Cell Proteomics.2013Otc;
You can visit "Citations Database" for more citations.
For more documents, please visit "Technical Support".
| Cat. No. A00626 | ||
|---|---|---|
| Size | Price | |
| 40 ug | $99.00 | |
