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Catalog Products » Antibodies » Epitope Tag Antibodies » AU1-tag Antibody, pAb, Rabbit

AU1-tag Antibody, pAb, Rabbit

AU1-tag Antibody, PAb, Rabbit

Lane 1-3: AU1-tag fusion protein in 293 cell lysate (≈ 52 kD)
Lane 4: Negative 293 cell lysate (GenScript, M0032)
Primary Antibody: 2 µg/ml Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

AU1-tag Antibody, PAb, Rabbit

Western blot analysis of AU1 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628, 1 µg/ml) The signal was developed with IRDyTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-AU1-tag; Anti-AU1-tag
Description Rabbit Anti-AU1-tag Polyclonal Antibody is purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.
Host Species Rabbit
Conjugation Unconjugated
Immunogen AU1 epitope tag peptide DTYRYI conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes AU1 tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

AU1-tag Antibody, PAb, Rabbit
AU1-tag Antibody, PAb, Rabbit

Lane 1-3: AU1-tag fusion protein in 293 cell lysate (≈ 52 kD)
Lane 4: Negative 293 cell lysate (GenScript, M0032)
Primary Antibody: 2 µg/ml Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

AU1-tag Antibody, PAb, Rabbit
AU1-tag Antibody, PAb, Rabbit

Western blot analysis of AU1 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628, 1 µg/ml) The signal was developed with IRDyTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A00628
Size
Price
40 ug $99.00
 
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