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Lane 1-3: AU1-tag fusion protein in 293 cell lysate (≈ 52 kD)Lane 4: Negative 293 cell lysate (GenScript, M0032)Primary Antibody: 2 µg/ml Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)
Western blot analysis of AU1 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-AU1-tag Polyclonal Antibody (GenScript, A00628, 1 µg/ml)
The signal was developed with IRDyTM 800 Conjugated Goat Anti-Rabbit IgG.
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