|Synonyms||Rabbit Anti-E2-tag; Anti-E2-tag|
|Description||Rabbit Anti-E2-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.|
|Immunogen||E2 epitope tag peptide GVSSTSSDFRDR conjugated to KLH|
|Concentration||1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide.|
|Specificity||This Antibody recognizes E2 tagged fusion proteins.|
|Storage||The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: User-optimized
|Technical Manual||ELISA Protocol(pdf)|
Western Blot Protocol(pdf)
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