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Catalog Products » Antibodies » Epitope Tag Antibodies » E-tag Antibody, pAb, Rabbit

E-tag Antibody, pAb, Rabbit

E-tag Antibody, PAb, Rabbit

Lane 1-4: E-tag fusion protein in CHO cell lysate (≈ 52 kD)
Lane 5: Negative CHO cell lysate
Primary Antibody: 1 µg/ml Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631)
Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

E-tag Antibody, PAb, Rabbit

Western blot analysis of E-tag fusion protein using 1 µg/ml Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631)

The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

E-tag Antibody, PAb, Rabbit

Western blot analysis of E tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-E-tag; Anti-E-tag
Description Rabbit Anti-E-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1.0 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1.0 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes E tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

E-tag Antibody, PAb, Rabbit
E-tag Antibody, PAb, Rabbit

Lane 1-4: E-tag fusion protein in CHO cell lysate (≈ 52 kD)
Lane 5: Negative CHO cell lysate
Primary Antibody: 1 µg/ml Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631)
Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

E-tag Antibody, PAb, Rabbit
E-tag Antibody, PAb, Rabbit

Western blot analysis of E-tag fusion protein using 1 µg/ml Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631)

The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

E-tag Antibody, PAb, Rabbit
E-tag Antibody, PAb, Rabbit

Western blot analysis of E tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-E-tag Polyclonal Antibody (GenScript, A00631, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00098 Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb
1 mg
$79.00
A00874-40 E-tag Antibody [HRP], pAb, Rabbit
40 ug
$99.00
 


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Cat. No. A00631
Size
Price
40 ug $99.00
24 hour
 
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