×
Catalog Products » Antibodies » Epitope Tag Antibodies » KT3-tag Antibody, pAb, Rabbit

KT3-tag Antibody, pAb, Rabbit

KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3-tagged fusion protein using KT3-tag Antibody, pAb, Rabbit (GenScript, A00632, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3-tagged fusion protein using KT3-tag Antibody, pAb, Rabbit (GenScript, A00632, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3 tagged fusion proteins expressed in E. coli cell lysate using KT3-tag Antibody (GenScript, A00632, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-KT3-tag pAb;
Description Rabbit Anti-KT3-tag Polyclonal Antibody is supplied as a 40 μg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide. It is purified by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen KT3 epitope tag peptide KPPTPPPEPET conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes KT3 tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

KT3-tag Antibody, PAb, Rabbit
KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3-tagged fusion protein using KT3-tag Antibody, pAb, Rabbit (GenScript, A00632, 1 µg/ml)
The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000) and LumiSensorTM HRP Substrate Kit (GenScript, L00221).
Predicted Size: 52 kD
Observed Size: 52 kD

KT3-tag Antibody, PAb, Rabbit
KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3-tagged fusion protein using KT3-tag Antibody, pAb, Rabbit (GenScript, A00632, 1 µg/ml)
The signal was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)
Predicted Size: 52 kD
Observed Size: 52 kD

KT3-tag Antibody, PAb, Rabbit
KT3-tag Antibody, PAb, Rabbit

Western blot analysis of KT3 tagged fusion proteins expressed in E. coli cell lysate using KT3-tag Antibody (GenScript, A00632, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A00098 Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb
1 mg
$79.00
 


Notice there is no results found, you can visit "Citations Database" for other results.







For more documents, please visit "Technical Support".

Cat. No. A00632
Size
Price
40 ug $99.00
100 ug $199.00
 
*
*
*
*