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Catalog Products » Antibodies » THE™ Elite Antibodies » THE™ HA-tag mAb » Protein C -Tag Antibody (HPC4), pAb, Rabbit

Protein C -Tag Antibody (HPC4), pAb, Rabbit

Protein C -Tag Antibody (HPC4), PAb, Rabbit

Loading: Protein C tag fusion protein expressed in E. coli cell lysate
Antibody: Rabbit Anti-Protein C (HPC4)-tag Polyclonal Antibody (GenScript, A00637)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

Protein C -Tag Antibody (HPC4), PAb, Rabbit

Western blot analysis of Protein C tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Protein C-tag Polyclonal Antibody (GenScript, A00637, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-Protein C (HPC4)-tag pAb;
Description Protein C is a Vitamin K-dependent plasma zymogen that is activated by proteolytic cleavage of the thrombin-thrombomodulin complex to form an anticoagulant enzyme. Protein C Tag is a 12 amino acid sequence EDQVDPRLIDGK, which encodes residues 6 through 17 of the heavy chain of the protein.Protein C Tag Antibody is a useful tool in analysis of Protein C fusion proteins.
Genscript Rabbit Anti-Protein C (HPC4)-tag antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Protein C epitope tag peptide CEDQVDPRLIDGK conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02%sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity This Antibody recognizes Protein C tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Protein C -Tag Antibody (HPC4), PAb, Rabbit
Protein C -Tag Antibody (HPC4), PAb, Rabbit

Loading: Protein C tag fusion protein expressed in E. coli cell lysate
Antibody: Rabbit Anti-Protein C (HPC4)-tag Polyclonal Antibody (GenScript, A00637)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

Protein C -Tag Antibody (HPC4), PAb, Rabbit
Protein C -Tag Antibody (HPC4), PAb, Rabbit

Western blot analysis of Protein C tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Protein C-tag Polyclonal Antibody (GenScript, A00637, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Cat. No.
Product Name
Size
Price
Quantity
A01774-40 THETM Protein C Tag Antibody (HPC4), mAb, Mouse
40 ug
$170.00
A01774-100 THETM Protein C Tag Antibody (HPC4), mAb, Mouse
100 ug
$350.00
 


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Cat. No. A00637
Size
Price
40 ug $99.00
 
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