Catalog Products » Antibodies » Epitope Tag Antibodies » B-tag Antibody, pAb, Rabbit
B-tag Antibody, PAb, Rabbit

Lane 1-2: B-tag fusion protein in E. coli cell lysate (≈ 30 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: 1µg/ml Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

B-tag Antibody, PAb, Rabbit

Western blot analysis of B tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

B-tag Antibody, pAb, Rabbit

GenScript Rabbit Anti-B-tag Polyclonal Antibody recognizesB tagged fusion proteins.
A00638
$102.00

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Overview
Specificity GenScript Rabbit Anti-B-tag Polyclonal Antibody recognizesB tagged fusion proteins.
Host Species Rabbit
Immunogen B epitope tag peptide QYPALT conjugated to KLH
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.05-0.2 µg/ml
Western Blot 0.1-1.0 µg/ml
Western Blot 1:100-1:1,000

Properties
Form Liquid
Storage Buffer 1×PBS, pH 7.4, containing 0.02% sodium azide
Concentration 1 mg/ml
Storage Instructions The product remains stable for up to 1 year at -20 °C from the date of receipt. The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Purification Affinity chromatography
Isotype Rabbit IgG
Clonality Polyclonal
Clone ID Not applicable
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Examples
  • B-tag Antibody, PAb, Rabbit
  • B-tag Antibody, PAb, Rabbit

    Lane 1-2: B-tag fusion protein in E. coli cell lysate (≈ 30 kD)
    Lane 3: Negative E. coli cell lysate
    Primary antibody: 1µg/ml Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638)
    Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

  • B-tag Antibody, PAb, Rabbit
  • B-tag Antibody, PAb, Rabbit

    Western blot analysis of B tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Background
Target Background Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The B-tag is an epitope tag composed of a six-residue peptide, QYPALT, corresponding to a highly conserved region of VP7 core protein of the blue tongue virus.The GenScript Rabbit Anti-B-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic B-tag peptide conjugated to KLH.GenScript Rabbit Anti-B-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml.
Synonyms Rabbit Anti-B-tag;Anti-B-tag

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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