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Catalog Products » Antibodies » Epitope Tag Antibodies » B-tag Antibody, pAb, Rabbit

B-tag Antibody, pAb, Rabbit

B-tag Antibody, PAb, Rabbit

Lane 1-2: B-tag fusion protein in E. coli cell lysate (≈ 30 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: 1µg/ml Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

B-tag Antibody, PAb, Rabbit

Western blot analysis of B tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-B-tag; Anti-B-tag
Description Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The B-tag is an epitope tag composed of a six-residue peptide, QYPALT, corresponding to a highly conserved region of VP7 core protein of the blue tongue virus. The GenScript Rabbit Anti-B-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic B-tag peptide conjugated to KLH.
GenScript Rabbit Anti-B-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 μg aliquot at a concentration of 1 mg/ml.
Host Species Rabbit
Conjugation Unconjugated
Immunogen B epitope tag peptide QYPALT conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide
Specificity GenScript Rabbit Anti-B-tag Polyclonal Antibody recognizesB tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using the ONE-HOUR WesternTM Kit: For quick results, the GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for an 8 cm X 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

B-tag Antibody, PAb, Rabbit
B-tag Antibody, PAb, Rabbit

Lane 1-2: B-tag fusion protein in E. coli cell lysate (≈ 30 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: 1µg/ml Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

B-tag Antibody, PAb, Rabbit
B-tag Antibody, PAb, Rabbit

Western blot analysis of B tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-B-tag Polyclonal Antibody (GenScript, A00638, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Lee F.,et al. Presence of bluetongue virus in the marginal zone of the spleen in acute infected sheep.Vet Microbiol.2011Aug;152(1-2):96-100

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Cat. No. A00638
Size
Price
40 ug $99.00
24 hour
 
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