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Catalog Products » Antibodies » Epitope Tag Antibodies » SUMO-tag Antibody, pAb, Rabbit

SUMO-tag Antibody, pAb, Rabbit

SUMO-tag Antibody, PAb, Rabbit

Lane 1-2:SUMO-tag fusion protein in E. coli lysate (≈70 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00640, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SUMO-tag Antibody, PAb, Rabbit

Lane 1: His-SUMO-tag fusion protein expressed in E. coli cell lysate (≈35 kD)
Lane 2: Yeast cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SUMO-tag Antibody, PAb, Rabbit

Loading: SUMO-tag fusion protein expressed in E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)

Synonyms Rabbit Anti-SUMO-tag pAb;
Description Small ubiquitin-related modifier (SUMO) proteins are conjugated to numerous intracellular targets and serve to modulate protein interaction, localization, activity, and stability. SUMO (also known as 'Smt3' and 'sentrin' in other organisms) is linked to several different pathways, including nucleocytoplasmic transport. The attachment of SUMO to targets proteins is stimulated by (protein inhibitor of activated STATs PIAS) proteins that serve as E3-like ligases.
GenScript Rabbit Anti-SUMO-tag Polyclonal Antibody is developed in rabbit s using purified recombinant yeast SUMO protein.
Host Species Rabbit
Antigen Species Yeast
Conjugation Unconjugated
Immunogen Purified recombinant yeast SUMO
Purification Protein G chromatography

Subclass Rabbit IgG
Concentration 5 mg/ml in PBS, pH7.4, containing 0.02% sodium azide
Specificity Rabbit Anti-SUMO-tag Polyclonal Antibody specifically reacts with fusion proteins containing SUMO protein.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µl of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

SUMO-tag Antibody, PAb, Rabbit
SUMO-tag Antibody, PAb, Rabbit

Lane 1-2:SUMO-tag fusion protein in E. coli lysate (≈70 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00640, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SUMO-tag Antibody, PAb, Rabbit
SUMO-tag Antibody, PAb, Rabbit

Lane 1: His-SUMO-tag fusion protein expressed in E. coli cell lysate (≈35 kD)
Lane 2: Yeast cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

SUMO-tag Antibody, PAb, Rabbit
SUMO-tag Antibody, PAb, Rabbit

Loading: SUMO-tag fusion protein expressed in E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204)




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Cat. No. A00640
Size
Price
500 ug $599.00
24 hour
 
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