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SUMO-tag Antibody, pAb, Rabbit

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Lane 1-2：SUMO-tag fusion protein in E. coli lysate (≈70 kD)
Lane 3: Negative E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00640, 1:10,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Lane 1: His-SUMO-tag fusion protein expressed in E. coli cell lysate (≈35 kD)
Lane 2: Yeast cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640, 1:1,000)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Loading: SUMO-tag fusion protein expressed in E. coli cell lysate
Primary antibody: Rabbit Anti-SUMO-tag Polyclonal Antibody (GenScript, A00640)
The result was developed with One-Step Western^TM Basic Kit (GenScript, L00204)

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Overview

Synonyms	Rabbit Anti-SUMO-tag pAb;
Description	Small ubiquitin-related modifier (SUMO) proteins are conjugated to numerous intracellular targets and serve to modulate protein interaction, localization, activity, and stability. SUMO (also known as 'Smt3' and 'sentrin' in other organisms) is linked to several different pathways, including nucleocytoplasmic transport. The attachment of SUMO to targets proteins is stimulated by (protein inhibitor of activated STATs PIAS) proteins that serve as E3-like ligases. GenScript Rabbit Anti-SUMO-tag Polyclonal Antibody is developed in rabbit s using purified recombinant yeast SUMO protein.
Host Species	Rabbit
Antigen Species	Yeast
Conjugation	Unconjugated
Immunogen	Purified recombinant yeast SUMO
Purification	Protein G chromatography

Properties

Subclass	Rabbit IgG
Concentration	5 mg/ml in PBS, pH7.4, containing 0.02% sodium azide
Specificity	Rabbit Anti-SUMO-tag Polyclonal Antibody specifically reacts with fusion proteins containing SUMO protein.
Storage	The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note	Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Applications

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µl of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)