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Catalog Products » Antibodies » Epitope Tag Antibodies » tag-100-tag Antibody, pAb, Rabbit

tag-100-tag Antibody, pAb, Rabbit

Tag-100-tag Antibody, PAb, Rabbit

Loading: tag-100-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 µg/ml Rabbit Anti-tag-100-tag Polyclonal Antibody (GenScript, A00677)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

Tag-100-tag Antibody, PAb, Rabbit

Western blot analysis of Tag-100 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Tag-100-tag Polyclonal Antibody (GenScript, A00677, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-tag-100-tag pAb;
Description Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. Tag-100 tag is an epitope tag composed of a 12-residue peptide, EETARFQPGYRS, derived from the C-termini of mammalian MAPK/ERK kinases.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Tag-100-tag peptide EETARFQPGYRS conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes Tag-100 tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (GenScript, L00204C) is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Tag-100-tag Antibody, PAb, Rabbit
Tag-100-tag Antibody, PAb, Rabbit

Loading: tag-100-tag fusion protein expressed in E. coli cell lysate
Antibody: 1 µg/ml Rabbit Anti-tag-100-tag Polyclonal Antibody (GenScript, A00677)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

Tag-100-tag Antibody, PAb, Rabbit
Tag-100-tag Antibody, PAb, Rabbit

Western blot analysis of Tag-100 tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-Tag-100-tag Polyclonal Antibody (GenScript, A00677, 1 µg/ml) The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


IK Wower, et al. Requirements for resuming translation in chimeric transfer-messenger RNAs of Escherichia coli and Mycobacterium tuberculosis. BMC Mol Biol. 2014Sep.;15(1):19

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Cat. No. A00677
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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