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Catalog Products » Antibodies » Epitope Tag Antibodies » Universal-tag Antibody, pAb, Rabbit

Universal-tag Antibody, pAb, Rabbit

Universal-tag Antibody, PAb, Rabbit

Universal-tag Antibody, PAb, Rabbit

Western blot analysis of Universal tagged fusion proteins expressed in E. coli cell lysate using Universal-tag Antibody (GenScript, A00680, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti Universal-tag pAb; Anti Universal-tag pAb
Description Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. Universal-tag is an epitope tag composed of a six-residue peptide, HTTPHH. GenScript Rabbit Anti-Universal-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Universal epitope tag peptide HTTPHH conjugated to KLH
Purification Immunoaffinity-purified

Subclass Rabbit IgG
Concentration 1 mg/ml in PBS, pH7.4, containing 0.02% sodium azide
Specificity This Antibody recognizes Universal tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit:
For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Universal-tag Antibody, PAb, Rabbit
Universal-tag Antibody, PAb, Rabbit

Universal-tag Antibody, PAb, Rabbit
Universal-tag Antibody, PAb, Rabbit

Western blot analysis of Universal tagged fusion proteins expressed in E. coli cell lysate using Universal-tag Antibody (GenScript, A00680, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A00680
Size
Price
40 ug $99.00
100 ug $199.00
24 hour
 
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