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Lane 1-2: MAT-tag fusion protein in E. coli lysateLane 3: E. coli lysate negative controlPrimary antibody: 2 μg/ml Rabbit Anti-MAT-tag Polyclonal Antibody (GenScript, A00681)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)
Western blot analysis of MAT tagged fusion proteins expressed in E. coli cell lysate using MAT-tag Antibody (GenScript, A00681, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.
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