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Catalog Products » Antibodies » Epitope Tag Antibodies » MAT-tag Antibody, pAb, Rabbit

MAT-tag Antibody, pAb, Rabbit

MAT-tag Antibody, PAb, Rabbit

Lane 1-2: MAT-tag fusion protein in E. coli lysate
Lane 3: E. coli lysate negative control
Primary antibody: 2 μg/ml Rabbit Anti-MAT-tag Polyclonal Antibody (GenScript, A00681)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

MAT-tag Antibody, PAb, Rabbit

Western blot analysis of MAT tagged fusion proteins expressed in E. coli cell lysate using MAT-tag Antibody (GenScript, A00681, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti-MAT-tag pAb; Anti-MAT-tag pAb
Description Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The MAT tag (Metal Affinity Tag) is a peptide tag that binds to transition metals such as nickel and cobalt. This tag allows the purification of MAT fusion proteins using immobilized metal affinity chromatography. GenScript Rabbit Anti-MAT-tag polyclonal antibody is developed in rabbit using a synthetic MAT tag peptide conjugated to KLH.
Rabbit Anti-MAT-tag polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Synthetic peptide conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol, and 0.02% sodium azide
Specificity This Antibody recognizes MAT tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Western blot using the GenScript ONE-HOUR WesternTM Kit: For quick results, we recommend the GenScript ONE-HOUR WesternTM Kit (L00204C). 15 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

MAT-tag Antibody, PAb, Rabbit
MAT-tag Antibody, PAb, Rabbit

Lane 1-2: MAT-tag fusion protein in E. coli lysate
Lane 3: E. coli lysate negative control
Primary antibody: 2 μg/ml Rabbit Anti-MAT-tag Polyclonal Antibody (GenScript, A00681)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

MAT-tag Antibody, PAb, Rabbit
MAT-tag Antibody, PAb, Rabbit

Western blot analysis of MAT tagged fusion proteins expressed in E. coli cell lysate using MAT-tag Antibody (GenScript, A00681, 1 µg/ml)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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Cat. No. A00681
Size
Price
40 ug $99.00
24 hour
 
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