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Catalog Products » Antibodies » Epitope Tag Antibodies » RFP-tag Antibody, pAb, Rabbit

RFP-tag Antibody, pAb, Rabbit

RFP-tag Antibody, PAb, Rabbit

Loading: RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204).

RFP-tag Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

RFP-tag Antibody, PAb, Rabbit

Western blot analysis of red fluorescent protein variants (mCherry, tdTomato, DsRed, DsRed2, mOrange)
using RFP-tag Antibody, pAb, Rabbit (GenScript, A00682, 1 µg/ml)

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

Synonyms Rabbit Anti RFP-tag pAb; Anti RFP-tag pAb
Description Well-characterizated antibodies against short-sequence epitope tags are common in the study of protein expression in systems. RFP-tags are composed of 15-residue peptides, VNGHEFEIEGEGEGR, derived from amino acids 22-36 of red fluorescent protein (RFP) from the Discosoma sea anemone.
Host Species Rabbit
Conjugation Unconjugated
Immunogen RFP epitope tag peptide VNGHEFEIEGEGEGR conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity GenScript RFP-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing RFP-tags and RFP variants, such as mCherry, tdTomato, DsRed, DsRed2, mOrange. The antibody does not react with GFP or GFP-tagged proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

RFP-tag Antibody, PAb, Rabbit
RFP-tag Antibody, PAb, Rabbit

Loading: RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The result was developed with One-Step WesternTM Basic Kit (GenScript, L00204).

RFP-tag Antibody, PAb, Rabbit
RFP-tag Antibody, PAb, Rabbit

Lane 1: Marker
Lane 2-5: 40 ng, 8 ng, 1.6 ng, 0.8 ng RFP-tag fusion protein expressed in E. coli cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-RFP-tag Polyclonal Antibody (GenScript, A00682)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG

RFP-tag Antibody, PAb, Rabbit
RFP-tag Antibody, PAb, Rabbit

Western blot analysis of red fluorescent protein variants (mCherry, tdTomato, DsRed, DsRed2, mOrange)
using RFP-tag Antibody, pAb, Rabbit (GenScript, A00682, 1 µg/ml)

The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.


Devers EA.,et al. An endogenous artificial microRNA system for unraveling the function of root endosymbioses related genes in Medicago truncatula.BMC Plant Biol.2013May;13:82

Daniel Nilsson and Per Sunnerhagen..,et al. Cellular stress induces cytoplasmic RNA granules in fission yeast.RNA.2011Jan;17(1):120 - 133

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Cat. No. A00682
Size
Price
40 ug $99.00
200 ug $349.00
100 ug $199.00
24 hour
 
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