TAP-tag Antibody, pAb, Rabbit Antibody

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Overview

Synonyms	Rabbit Anti-TAP-tag pAb; Anti-TAP-tag pAb
Description	Well-characterized antibodies against short-sequence epitope tags are common in the study of protein expression in several different expression systems. The TAP (tandem affinity purification)-tag allows rapid purification of complexes from a relatively small number of cells even if the researcher does not have prior knowledge of the complexs composition, activity, or function. Combined with mass spectrometry, the TAP-tag strategy enables the identification of proteins interacting with a given target protein¹. GenScript Rabbit Anti-TAP-tag Polyclonal Antibody is generated against the common tag, which is composed of a 21-residue peptide, CSSGALDYDIPTTASENLYFQ, derived from the C-terminus of the TAP-tag construct after TEV cleavage.
Host Species	Rabbit
Conjugation	Unconjugated
Immunogen	Peptide CSSGALDYDIPTTASENLYFQ conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Specificity	Rabbit Anti-TAP-tag Polyclonal Antibody specifically reacts with fusion proteins containing TAP epitope tag.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Western blot using ONE-HOUR Western^TM Kit: For quick results, GenScript ONE-HOUR Western^TM Kit L00204C is recommended. 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm x 8 cm membrane.
immunoprecipitation (IP):2-10 µg/mg of lysate
Other applications: user-optimized

Technical Manual

ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)
IF & ICC Protocol(pdf)

Examples

Western blot analysis of TAP-tag fusion protein expressed in E. coli cell lysate, using 1 μg/ml Rabbit Anti-TAP-tag Polyclonal Antibody (GenScript, A00683).
The signal was developed with One-Step Western^TM Complete Kit (GenScript, L00204C)

Western blot analysis of immunoprecipitates from yeast lysate expressing Gcn5-TAP using TAP-tag Antibody (GenScript, A00683).
IP: Immunprecipitation with TAP-tag Antibody (GenScript, A00683)
NB: Not bound fraction
Input control: Input control material for yeast lysate expressing Gcn5-TAP protein

Related Products

	Cat. No.	Product Name	Price
	A01435-40	TAP-tag Antibody [HRP], pAb, Rabbit - 40 ug	$99.00
	A01435-100	TAP-tag Antibody [HRP], pAb, Rabbit - 100 ug	$199.00

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OravcovÁ M.,et al. Fission Yeast CSL Proteins Function as Transcription Factors.PLOS ONE.2013Mar;8(3):e59435

Christopher J. Shoemaker and Rachel Green..,et al. Kinetic Analysis Reveals The Ordered Coupling Of Translation Termination And Ribosome Recycling In Yeast.Proc Natl Acad Sci U S A.2011Dec;

Dieppedale J.,et al. Possible links between stress defense and the tricarboxylic acid cycle in Francisella pathogenesis.Mol Cell Proteomics.2013May;

Chymkowitch P.,et al. Cdc28 Kinase Activity Regulates The Basal Transcription Machinery At A Subset Of Genes.Proc Natl Acad Sci U S A.2012Jun;109(26):10450 - 10455

Rojas DA.,et al. Rrn7 Protein, an RNA Polymerase I Transcription Factor, Is Required for RNA Polymerase II-dependent Transcription Directed by Core Promoters with a HomolD Box Sequence.J Biol Chem.2011Jul;286(30):26480 - 26486

Lev I.,et al. Reverse PCA, a Systematic Approach for Identifying Genes Important for the Physical Interaction between Protein Pairs.PLoS Genet.2013Otc;9(10):e1003838

Chen X.,et al. Cell cycle regulation of DNA double-strand break end resection by Cdk1-dependent Dna2 phosphorylation.Nat Struct Mol Biol.2011Aug;18(9):1015-9

Choi JS.,et al. Caloric restriction improves efficiency and capacity of the mitochondrial electron transport chain in Saccharomyces cerevisiae.Biochem Biophys Res Commun.2011Jun;409(2):308-14

Atencio D, et al. The Yeast Ess1 Prolyl Isomerase Controls Swi6 and Whi5 Nuclear Localization. G3 (Bethesda). 2014Mar.;4(3):523-537

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TAP-tag Antibody, pAb, Rabbit

Overview

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Examples

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