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Catalog Products » Antibodies » Epitope Tag Antibodies » SNAP-tag Antibody, pAb, Rabbit

SNAP-tag Antibody, pAb, Rabbit

Synonyms Anti SNAP-tag polyclonal antibody;
Description Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The SNAP-tag is a self-labeling protein tag that forms a highly stable, covalent thioether bond with fluorophores and other substituted groups on its benzyl guanine substrate. The expressed SNAP-tag fusion proteins can be used in many different assay formats depending upon the labeling substrate employed.
GenScript Rabbit Anti-SNAP-tag Polyclonal Antibody is developed in rabbit hosts using purified recombinant SNAP-tag fusion protein. This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Purified recombinant SNAP-tag fusion protein
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
Reconstitution Reconstitute the lyophilized antibody with deionized water to the final concentration of 0.5 mg/ml.
Specificity Rabbit Anti-SNAP-tag Polyclonal Antibody specifically reacts with fusion proteins containing the SNAP-tag.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

SNAP-tag Antibody, PAb, Rabbit
SNAP-tag Antibody, PAb, Rabbit

Lane 1-2: SNAP fusion protein in 293 cell lysate (≈ 28 kD)
Lane 3: Negative 293 cell lysate
Primary antibody: 1µg/ml Rabbit Anti-SNAP-tag Polyclonal Antibody (GenScript, A00684)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Foraker AB.,et al. Clathrin Promotes Centrosome Integrity In Early Mitosis Through Stabilization Of Centrosomal Ch-Tog.J Cell Biol.2012Aug;198(4):591 - 605

Ichikawa M.,et al. Recombinant human cytoplasmic dynein heavy chain 1 and 2: Observation of dynein-2 motor activity in vitro.FEBS Lett.2011Aug;585(15):2419-23

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Cat. No. A00684
Size
Price
40 ug $99.00
24 hour
 
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