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Catalog Products » Other Products » β-Amyloid Antibody (25-35), pAb, Rabbit

β-Amyloid Antibody (25-35), pAb, Rabbit

Synonyms Rabbit Anti β-Amyloid (25-35) pAb; Anti β-Amyloid (25-35) pAb
Description A number of mutations, identified in the gene encoding the β-amyloid precursor protein (βAPP), have been linked to early-onset Familial Alzheimers Disease. βAPP is cleaved sequentially by the proteolytic enzymes β-secretase and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42(43) and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides can bind to scavenger receptors and the receptor for advanced glycation endproducts. Aβ peptides are degraded either via a reuptake mechanism followed by endosomal degradation or by an extracellular insulin-degrading enzyme. Extracellular accumulation of Aβ leads to formation of aggregates, fibrils, and eventually amyloid deposits called neuritic plaques, a hallmark of Alzheimer’s disease. β-Amyloid antibodies and peptides have been developed as tools for elucidating the biology of Alzheimers disease.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic peptide corresponding to amino acids 25-35 of human β-Amyloid conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration See label for antibody concentration
Buffer: PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-β-Amyloid (25-35) Polyclonal Antibody detects human β-amyloid 25-35 and β-amyloid 1-40 and does not react with β-amyloid 1-42.
Species Reactivity Human
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2.0 µg/ml
Other applications: user-optimized

  • β-Amyloid Antibody (25-35), PAb, Rabbit
  • β-Amyloid Antibody (25-35), PAb, Rabbit

    Western blot analysis of human β-Amyloid 1-40 peptide
    using Rabbit Anti β-Amyloid (25-35) Polyclonal Antibody (GenScript, A00687)
    The signal was developed with Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098)
    and LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)


Liyun Jia.,et al. Berberine suppresses amyloid-beta-induced inflammatory response in microglia by inhibiting nuclear factor-kappaB and mitogen-activated protein kinase signalling pathways.J Pharm Pharmacol.2012Otc;64(10):1510-21

Chu YF.,et al. Crude caffeine reduces memory impairment and amyloid β 1-42 levels in an Alzheimer's mouse model.Food Chem.2012Dec;135(3):2095-102

Anil K. Mantha.,et al. Proteomic study of amyloid beta (25–35) peptide exposure to neuronal cells: Impact on APE1/Ref‐1's protein–protein interaction.J Neurosci Res.2012Jun;90(6):1230-9

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Cat. No. A00687
Size
Price
40 ug $99.00