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Catalog Products » Antibodies » Primary Antibodies » Alzheimer's Disease Antibody list » Amyloid Precursor Protein Antibody (671-685), pAb, Rabbit

Amyloid Precursor Protein Antibody (671-685), pAb, Rabbit

Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

Lane 1-2: Recombinant human APP expressed in E. coli cell lysate
Lane 3: Negative E. coli cell lysate
Primary Antibody: 1µg/ml Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)
Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:5,000)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

Immunofluorescent analysis of human brain tissue slide using Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)

Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

Immunofluorescent analysis of human brain tissue slide using Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)

Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

Loading: Recombinant human APP expressed in E. coli cell lysate
Primary Antibody: Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)
The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)

*This product is under development*
Synonyms Rabbit Anti-APP (671-685) pAb;
Description Amyloid precursor protein (APP) is a type I integral membrane protein with a large N-terminal extracellular domain, a single transmembrane domain, and a short cytoplasmic tail. APP is found in three main isoforms: APP695, APP751, and APP770. APP can be sequentially cleaved by beta-secretase and gamma-secretase to release beta-amyloid, which is the main component of senile plaque in patient brains inflicted with Alzheimer’s disease.

GenScript Rabbit Anti-APP (671-685) Polyclonal Antibody is developed in rabbit using a synthetic peptide, MDAEFRHDSGYEVHH, corresponding to C terminal amino acids 671-685 of human APP, conjugated to KLH. GenScript Rabbit Anti-APP (671-685) Polyclonal Antibody is a valuable tool for scientists and neuropathologists who study the role of APP in Alzheimers’ disease. GenScript Rabbit Anti APP (671-685) Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography.
Host Species Rabbit
Antigen Species Human
Immunogen Synthetic peptide corresponding to amino acids 671-685 of Human Amyloid Precursor Protein conjugated to KLH
Purification Immunoaffinity chromatography

Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-APP (671-685) Polyclonal Antibody can detect both human recombinant APP and endogenous APP from cell lysates and tissue slides and mouse APP in brain tissue lysate.
Species Reactivity Human and mouse
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Immunofluorescence: 5-20 µg/ml
Western Blot Using GenScript ONE-HOUR WesternTM Kit: For quick results, we recommend the GenScript ONE-HOUR WesternTM Complete Kit (Rabbit) (L00204C). 10 µg of this antibody is mixed with 10 ml of WB solution for 8 cm X 8 cm membrane.
Other applications: user-optimized

  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit
  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

    Lane 1-2: Recombinant human APP expressed in E. coli cell lysate
    Lane 3: Negative E. coli cell lysate
    Primary Antibody: 1µg/ml Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)
    Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:5,000)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit
  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

    Immunofluorescent analysis of human brain tissue slide using Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)

  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit
  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

    Immunofluorescent analysis of human brain tissue slide using Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)

  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit
  • Amyloid Precursor Protein Antibody (671-685), PAb, Rabbit

    Loading: Recombinant human APP expressed in E. coli cell lysate
    Primary Antibody: Rabbit Anti-APP (671-685) Polyclonal Antibody (GenScript, A00695)
    The result was developed with One-Step WesternTM Complete Kit (Rabbit) (GenScript, L00204C)




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