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Catalog Products » Antibodies » THE™ Elite Antibodies » THETM c-Myc Tag Antibody, mAb, Mouse

THETM c-Myc Tag Antibody, mAb, Mouse

 THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

20 μg Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704) and half of th IP was loaded on the gel.
The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232)

 THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using
THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704)

 THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.

Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 µg/ml) in assay.

The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

 THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.

A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)

The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG

 THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.

A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)

The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG

Synonyms Mouse Anti-c-Myc-tag mAb;
Description The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays.
Host Species Mouse
Conjugation Unconjugated
Immunogen A synthetic peptide EQKLISEEDL coupled to KLH
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells
Purification Protein A affinity column
Key Features
  • GenScript THETM c-Myc Tag Antibody, mAb, Mouse has high sensitivity, better than the antibody on the market.
  • GenScript THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) can recognize N-terminal, C-terminal and internal c-Myc-tag in fusion proteins.

  • Subclass IgG2a, k
    Clone ID 2G8D5
    Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide
    Reconstitution Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml.
    Specificity THETM c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates.
    Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05 µg/ml-0.2 µg/ml
    Western blot: 0.05 µg/ml-0.5 µg/ml
    Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 0.9 µg of this antibody is mixed with diluted WB solution (dilute 1.5 ml WB with 1.5 ml of PBST).
    Immunoprecipitation (IP): 1 µg/ml
    Immunofluorescent staining: 1 µg/ml
    Other applications: user-optimized
    Technical Manual ELISA Protocol(pdf)
    Western Blot Protocol(pdf)
    FACS Protocol(pdf)
    IHC Protocol(pdf)
    IP Protocol(pdf)
    IF & ICC Protocol(pdf)

     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse
     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

    20 μg Multiple Tag Cell Lysate (M0100) was used for the IP using THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704) and half of th IP was loaded on the gel.
    The Western blot was performed by One-Step IP-Western Kit (Mouse) (L00232)

     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse
     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

    Immunofluorescence analysis of 293 cells transfected with c-Myc tag protein using
    THETM Anti-c-Myc-tag Monoclonal Antibody (Mouse) (GenScript, A00704)

     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse
     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

    Western blot discovers working concentration comparison of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with Mouse Anti-c-Myc mAb (9E10), showing GenScript A00704 posses excellent performance in WB assay with surprising antibody dilution.

    Both c-Myc antibodies starting dilution are from 1:1 000 (equivalent to 1.0 µg/ml) in assay.

    The signal was developed with IRDye™ 800 Conjugated affinity Purified Goat Anti-Mouse IgG.

     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse
     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

    Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.

    A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
    B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
    C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)

    The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG

     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse
     THE<sup>TM</sup> C-Myc Tag Antibody, MAb, Mouse

    Sensitivity analysis of THETM c-Myc Tag Antibody, mAb, Mouse (Cat.No. A00704) with series of loading amounts and antibody concentration.

    A: 0.2 µg/ml c-Myc Tag Antibody (equivalent to 1:5 000 diluted from 1.0 mg/ml)
    B: 0.1 µg/ml c-Myc Tag Antibody (equivalent to 1:10 000 diluted from 1.0 mg/ml)
    C: 0.05 µg/ml c-Myc Tag Antibody (equivalent to 1:20 000 diluted from 1.0 mg/ml)

    The signal was developed with IRDyeTM 800 Conjugated affinity Purified Goat Anti-Mouse IgG


    Cat. No.
    Product Name
    Size
    Price
    Quantity
    A00172-40 c-Myc-tag Antibody, pAb, Rabbit
    40 ug
    $99.00
    A00173-100 c-Myc-tag Antibody [HRP], pAb, Rabbit
    100 ug
    $199.00
    A00173-40 c-Myc-tag Antibody [HRP], pAb, Rabbit
    40 ug
    $99.00
    A00704-100 THETM c-Myc Tag Antibody, mAb, Mouse
    100 ug
    $175.00
    A00864-40 THETM c-Myc Antibody [Biotin], mAb, Mouse
    40 ug
    $74.00
    A00864-200 THETM c-Myc Antibody [Biotin], mAb, Mouse
    200 ug
    $333.00
    A00863-100 THETM c-Myc Antibody [HRP], mAb, Mouse
    100 ug
    $180.00
     
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    You can visit "Citations Database" for more citations.







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    Cat. No. A00704
    Size
    Price
    40 ug $69.00
    100 ug $175.00
    24 hour
     
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