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CK1 Antibody, mAb, Mouse

*This product has been discontinued! *
Synonyms Mouse Anti-CK1 mAb;
Description CK1 (also designated Cytokeratin 1 or KRT1), with 644-amino acid protein (about 70,000 Da), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Host Species Mouse
Antigen Species Human
Immunogen Ni-NTA purified truncated recombinant CK1 expressed in E. coli strain BL21 (DE3)

Subclass IgG1
Clone ID A00814.01
Concentration 1 mg/ml in 10 mM PBS (pH 7.6) and 0.02% sodium azide
Specificity Truncated CK1 recombinant protein (WB), human CK1 recombinant protein (ELISA), human liver tissue / colon carcinoma / lung carcinoma / esophagus tissue (IHC)
Storage This product remains stable for one to two weeks if stored at 2°C to 8°C. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. Use before is one year from the date of receipt.

Western blot: 1:500-1:2,000
IHC(P): 1:500-1:2,000
IHC(F): 1:500-1:2,000
ELISA: proposed dilution 1:10,000
Determining optimal working dilutions by titration test
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

Ricardo G. Espinola, Robin A. Pixley, Berhane Ghebrehiwet Blood (ASH Annual Meeting Abstracts), Nov 2005; 106: 2666

Bauer K.,et al. P-cadherin controls the differentiation of oral keratinocytes by regulating cytokeratin 1/10 expression via C/EBP-beta-mediated signaling.Differentiation.2012Dec;84(5):345-54

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