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HPRT Antibody, mAb, Mouse

*This product has been discontinued! *
Synonyms Mouse Anti-HPRT mAb;
Description The HPRT1 gene provides instructions for making an enzyme called hypoxanthine phosphoribosyltransferase 1. This enzyme allows cells to recycle purines, some of the building blocks of DNA and its chemical cousin RNA. The enzyme hypoxanthine-guanine phosphoribosyltrasferase (E.C., HPRT) plays a crucial role in uric acid synthesis and purine metabolism. This enzyme catalyzes the conversion of hypoxanthine and guanine to inosine monophosphate (IMP) and guanosine monophosphate (GMP), respectively, and uses phosphoribosylpyrophosphate (PRPP) as a cosubstrate and as a source of energy. This pathway is also known as the purine salvage pathway because it allows cells to reuse purine compounds to build DNA and RNA.
Host Species Mouse
Antigen Species Human
Conjugation Unconjugated
Immunogen Ni-NTA purified truncated recombinant HPRT expressed in E. coli strain BL21 (DE3)

Subclass IgG2b
Clone ID A00842.01
Concentration Ascitic fluid containing 0.03% sodium azide
Specificity Truncated HPRT recombinant protein (WB), HPRT recombinant protein (ELISA)
Storage This product remains stable for one to two weeks if stored at 2°C to 8°C. Aliquot and store at -20°C or below for longer periods. Avoid repeated freezing and thawing cycles. Use within one year of the delivery date.

Western blot: 1:500-1:2,000
ELISA: 1:10,000
Determine optimal working dilutions by titration test.
Technical Manual ELISA Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

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24 hour