THETM c-Myc Antibody [HRP], mAb, Mouse
| Synonyms | Mouse Anti-c-Myc [HRP] mAb; |
| Description | GenScript THETM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins. |
| Host Species | Mouse |
| Conjugation | Peroxidase (Horseradish) |
| Immunogen | A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH |
| Fusion Partner | Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells. |
| Purification | Protein A affinity column |
| Subclass | Mouse IgG2a |
| Clone ID | 2G8D5 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml. |
| Specificity | GenScript THETM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05–0.2 µg/ml Western blot: 0.1-1 µg/ml Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Other applications: user-optimized |
![THE<sup>TM</sup> C-Myc Antibody [HRP], MAb, Mouse](/product/documents/down?doc_name=3645_20071212213127.JPG&file=filecenter/document/3645_20071212213127.JPG "THE<sup>TM</sup> C-Myc Antibody [HRP], MAb, Mouse")
Lane 1 and 2: Multiple Tag Cell Lysate (GenScript, M0100)
Primary antibody: 0.1 μg/ml THETM Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) (GenScript, A00863)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)
| Cat. No. | Product Name | Quantity | Price |
|---|---|---|---|
A00864-200 |
THETM c-Myc Antibody [Biotin], mAb, Mouse - 200 ug |
$333.00 |
|
A00863-100 |
THETM c-Myc Antibody [HRP], mAb, Mouse - 100 ug |
$180.00 |
|
A00704-100 |
THETM c-Myc Tag Antibody, mAb, Mouse - 100 ug |
$175.00 |
|
A00704-40 |
THETM c-Myc Tag Antibody, mAb, Mouse - 40 ug |
$69.00 |
|
A00173-40 |
c-Myc-tag Antibody [HRP], pAb, Rabbit - 40 ug |
$99.00 |
|
A00172-40 |
c-Myc-tag Antibody, pAb, Rabbit - 40 ug |
$99.00 |
For more documents, please visit "Technical Support".
| Cat. No. A00863 | ||
|---|---|---|
| Size | Price | |
| 100 ug | $180.00 | |
