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THE™ c-Myc Antibody [HRP], mAb, Mouse

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Overview

Synonyms	Mouse Anti-c-Myc [HRP] mAb;
Description	GenScript THE^TM c-Myc Antibody [HRP], mAb, Mouse is immunoaffinity purified from mice ascites with protein A and then conjugated to horseradish peroxidase. Mouse Anti-c-Myc [HRP] Monoclonal Antibody is suitable for detecting the expression level of c-Myc in fusion proteins.
Host Species	Mouse
Conjugation	Peroxidase (Horseradish)
Immunogen	A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH
Fusion Partner	Spleen cells are fused with SP2/0-Ag14 mouse myeloma cells.
Purification	Protein A affinity column

Properties

Subclass	Mouse IgG2a
Clone ID	2G8D5
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	GenScript THE^TM c-Myc Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc-tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes the c-Myc gene in human cancers, such as Hela whole cell lysate.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05–0.2 µg/ml
Western blot: 0.1-1 µg/ml
Immunoprecipitation (IP): 1 µg/ml
Immunofluorescent staining: 1 µg/ml
Other applications: user-optimized

Examples

Lane 1 and 2: Multiple Tag Cell Lysate (GenScript, M0100)
Primary antibody: 0.1 μg/ml THE^TM Anti-c-Myc [HRP] Monoclonal Antibody (Mouse) (GenScript, A00863)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)

Related Products

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A00864-200	THE™ c-Myc Antibody [Biotin], mAb, Mouse - 200 ug	$333.00
A00863-100	THE™ c-Myc Antibody [HRP], mAb, Mouse - 100 ug	$180.00
A00704-100	THE™ c-Myc Tag Antibody, mAb, Mouse - 100 ug	$175.00
A00704-40	THE™ c-Myc Tag Antibody, mAb, Mouse - 40 ug	$69.00
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