THE™ GST Antibody, mAb, Mouse

Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

Cat. No.	A00865S
Size	10 μg Buy in bulk
Quantity
Price	$0.00


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Overview

Specificity	THE^TM Anti-GST Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged GST fusion proteins.
Host Species	Mouse
Immunogen	Recombinant GST protein (26 kD)

Properties

Concentration	1. 40 µg, 100 µg, 200 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. 2. 500 µg (GenScript, A00865), 0.5 mg/ml, lyophilized with PBS, pH 7.4, without sodium azide or carrier protein.
Reconstitution	Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.
Purification	Protein A affinity column
Clone ID	2F10B9

Background

Target Background

Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

Synonyms

Mouse Anti-GST monoclonal antibody;