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E-tag Antibody [HRP], pAb, Rabbit

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Overview

Synonyms	Rabbit Anti-E-tag [HRP] pAb;
Description	GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-E-tag [HRP] Polyclonal Antibody is suitable for detecting fusion proteins that contain E-tags.
Host Species	Rabbit
Conjugation	Peroxidase (Horseradish)
Immunogen	E epitope tag peptide GAPVPYPDPLEPR conjugated to KLH
Purification	Immunoaffinity chromatography

Properties

Subclass	Rabbit IgG
Concentration	0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution	Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Specificity	GenScript Anti-E-tag [HRP] Polyclonal Antibody recognizes E tagged fusion proteins.
Storage	The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized

Examples

Lane 1: E-tag fusion protein in Hela cell lysate (≈52 kD)
Lane 2: Negative Hela cell lysate
Primary antibody: 1 μg/ml Rabbit Anti-E-tag [HRP] Polyclonal Antibody (GenScript, A00874)
The signal was developed with LumiSensor^TM HRP Substrate Kit (GenScript, L00221V500)