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Catalog Products » Antibodies » Epitope Tag Antibodies » V5-tag Antibody [HRP], pAb, Rabbit

V5-tag Antibody [HRP], pAb, Rabbit

Synonyms Rabbit Anti-V5-tag [HRP] pAb;
Description GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. GenScript Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
Host Species Rabbit
Conjugation Horseradish Peroxidase (HRP)
Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
Purification Immunoaffinity chromatography

Subclass Rabbit IgG
Concentration 40μg:1 mg/ml in PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal.
100 μg:0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Specificity GenScript Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.1-1.0 µg/ml
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

  • V5-tag Antibody [HRP], PAb, Rabbit
  • V5-tag Antibody [HRP], PAb, Rabbit

    Western blot analysis of V5-tagged fusion protein using V5-tag Antibody [HRP], pAb, Rabbit (GenScript, A00877, 1 µg/ml)
    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221)
    Predicted Size: 52 kD
    Observed Size: 52 kD


Cat. No.
Product Name
Price
A00877-40
V5-tag Antibody [HRP], pAb, Rabbit - 40 ug
$99.00
A01724-40
THETM V5 Tag Antibody, mAb, Mouse - 40 ug
$119.00
A01724-100
THETM V5 Tag Antibody, mAb, Mouse - 100 ug
$219.00
A01724-200
THETM V5 Tag Antibody, mAb, Mouse - 200 ug
$395.00
A01733-100
THETM V5 Tag Antibody [HRP], mAb, Mouse - 100 ug
$239.00
A01735-100
THETM V5 Tag Antibody [Biotin], mAb, Mouse - 100 ug
$239.00
A00877-100
V5-tag Antibody [HRP], pAb, Rabbit - 100 ug
$199.00
A01803-100
THETM V5 Tag Antibody [iFluor 488], mAb, Mouse - 100 ug
$263.00
A01804-100
THETM V5 Tag Antibody [iFluor 555], mAb, Mouse - 100 ug
$263.00
A01805-100
THETM V5 Tag Antibody [iFluor 647], mAb, Mouse - 100 ug
$263.00
 
Tahir SA.,et al. Caveolin-1-LRP6 Signaling Module Stimulates Aerobic Glycolysis in Prostate Cancer.Cancer Res.2013Jan;

Zhang K.,et al. The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit.J Cell Biol.2013Mar;200(6):807 - 820

Dahl LD, et al. An EF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus. Cancer Cell Int. 2014Feb.;14(1):17

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For more documents, please visit "Technical Support".

Cat. No. A00877
Size
Price
40 ug $99.00
100 ug $199.00
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