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IDO Antibody, pAb, Rabbit

Synonyms Rabbit Anti-IDO pAb;
Description Indoleamine 2,3-dioxygenase (IDO) catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated in protection against intracellular and extracellular pathogens. GenScript Rabbit Anti-IDO Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide conjugated to KLH.
GenScript Rabbit Anti-IDO Polyclonal Antibody is highly purified from rabbit antiserum by Protein A.
Host Species Rabbit
Conjugation Unconjugated
Immunogen Synthetic peptide KPSKKKPTDGDKSE conjugated to KLH
Purification Protein A chromatography

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-IDO Polyclonal Antibody reacts with mouse IDO. It has not yet been tested with other species.
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.1-1.0 µg/ml
Western Blot Using the ONE-HOUR WesternTM Kit: For quick results, we recommend the GenScript ONE-HOUR WesternTM Kit (L00204C). Mix 10 µg of this antibody with 10 ml of WB solution for an 8 cm x 8 cm membrane.
Other applications: user-optimized
Technical Manual ELISA Protocol(pdf)
Western Blot Protocol(pdf)
FACS Protocol(pdf)
IHC Protocol(pdf)
IP Protocol(pdf)

IDO Antibody, PAb, Rabbit
IDO Antibody, PAb, Rabbit

Lane 1: EasyWestern Protein Standard (GenScript, MM0908)
Lane 2: 50 μg Mouse heart tissue lysate
Primary antibody: 1 μg/ml Rabbit Anti-IDO Polyclonal Antibody (GenScript, A00887)
Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098, 1:10,000)

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Cat. No. A00887
40 ug $99.00
24 hour