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Catalog Products » Antibodies » Primary Antibodies » Phospho-Specific Antibodies » Tau Antibody (Phospho-Ser400), pAb, Rabbit

Tau Antibody (Phospho-Ser400), pAb, Rabbit

Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit

Western blot analysis of mouse brain tissue lysates using
Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody (GenScript, A00898)

Lane 1: Rabbit IgG Control
Lane 2: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody
Lane 3: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with non-phoshpo-peptide
Lane 4: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with phoshpo-peptide
Lane 5: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with generic phosphoserine containing peptide

Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098)

The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit

Western blot analysis of rat brain lysates using 1 µg/ml GenScript Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody (GenScript, A00898)
The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.

*This product is under development*
Synonyms Rabbit Anti-Tau (Phospho-Ser400) pAb;
Description Tau is a microtubule-associated phosphoprotein (MAP), localized in neuronal axons. It promotes tubulin polymerization and stabilizes microtubules. Tau proteins constitute a family of six isoforms, which ranging from 352 to 441 amino acids. The tau variants differ from each other by the presence of either three or four repeat-regions in the carboxy-terminal part of the molecule and the absence or presence of one or two inserts in the amino-terminal portion.
Tau is hyperphosphorylated by ERK, GSK-3, TPKII, and CDK5. At least thirty phosphorylation sites have been described, including Thr39, Ser46, Thr50, Thr69, Thr153, Thr175, Thr181, Ser198, Ser199, Ser202, Thr205, Ser208, Ser210, Thr212, Ser214, Thr217, Thr231, Ser235, Ser237, Ser241, Ser262, Ser285, Ser305, Ser324, Ser352, Ser356, Ser396, Ser400, Thr403, Ser404, Ser409, Ser412, Ser413, Ser416, and Ser422. Specifically, TPKII phosphorylates serines 202 and 404. GSK-3β transfection phosphorylates serines 199, 202, 235, 396, 404, and 413, and threonines 205 and 231. These sites are among the major abnormal phosphorylation sites of Tau. Phosphorylation on these sites reduces the ability of a given Tau species to promote microtubule self-assembly. Hyperphosphorylated Tau is the major protein of the paired helical filaments (PHFs), which make up the pathological neurofibrillary tangles of Alzheimer’s disease (AD). The PHFs are also found in the lesions of other central nervous system disorders.
GenScript Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody is developed in rabbit using a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding serine 400 of human Tau.
Host Species Rabbit
Antigen Species Human
Conjugation Unconjugated
Immunogen Synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding serine 400 of human Tau
Purification The antibody is purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide and cross-adsorbed with the corresponding non-phosphopeptide.

Subclass Rabbit IgG
Concentration 0.5 mg/ml in PBS, pH 7.4, containing 30% glycerol and 0.02% sodium azide
Specificity GenScript Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody detects endogenous levels of Tau only when phosphorylated at serine 400.
Species Reactivity Human, mouse, and rat
Storage The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-2 µg/ml
Other applications: user-optimized

  • Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit
  • Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit

    Western blot analysis of mouse brain tissue lysates using
    Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody (GenScript, A00898)

    Lane 1: Rabbit IgG Control
    Lane 2: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody
    Lane 3: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with non-phoshpo-peptide
    Lane 4: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with phoshpo-peptide
    Lane 5: Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody pre-incubated with generic phosphoserine containing peptide

    Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00098)

    The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500)

  • Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit
  • Tau Antibody (Phospho-Ser<sup>400</sup>), PAb, Rabbit

    Western blot analysis of rat brain lysates using 1 µg/ml GenScript Rabbit Anti-Tau (Phospho-Ser400) Polyclonal Antibody (GenScript, A00898)
    The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.




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